John C Ransom

US Patent:

7858040, Dec 28, 2010

Filed:

May 7, 2004

Appl. No.:

10/841810

Inventors:

Alex Okun - San Diego CA, US
Teresa A. Bennett - San Diego CA, US
Andrew Beernink - San Diego CA, US
David J. Sieg - Escondido CA, US
John T. Ransom - Encinitas CA, US

Assignee:

Saryna Biotechnologies LLC - San Diego CA

International Classification:

B01L 3/02

US Classification:

422100, 436 49, 7386401

Abstract:

A system and method of mixing and injecting discrete sample mixtures into a flow cytometer or other sample analysis apparatus may generally comprise a sample injection guide coupling a liquid handling apparatus with a sample analysis apparatus and facilitating injection of discrete sample mixtures into a fluidic system of the apparatus.

US Patent:

20050009060, Jan 13, 2005

Filed:

May 7, 2004

Appl. No.:

10/841410

Inventors:

Andrew Beernink - San Diego CA, US
Teresa Bennett - San Diego CA, US
Alex Okun - San Diego CA, US
Juan Ballesteros - San Diego CA, US
John Ransom - Encinitas CA, US

International Classification:

C12Q001/68
G01N001/30
G01N033/48

US Classification:

435006000, 435040500

Abstract:

The present disclosure provides systems for multiplexed multitarget screening of cell populations having one or more wild type or mutated ligand targets and measuring cell responses to ligands using high throughput screening techniques, including flow cytometry (FCM). The method includes the steps of: 1) developing cell populations to be screened; 2) staining cell populations using one or more fluorochromes to yield a distinct excitation/emission signature for each cell population; 3) combining labelled cell populations into a single mixed suspension; 4) analyzing populations to resolve them on the basis of their unique signature; and 5) resolving individual populations and deconvoluting data to extract meaningful information about populations.

US Patent:

20050021241, Jan 27, 2005

Filed:

May 7, 2004

Appl. No.:

10/841974

Inventors:

Juan Ballesteros - San Diego CA, US
Teresa Bennett - San Diego CA, US
Alex Okun - San Diego CA, US
Andrew Beernink - San Diego CA, US
John Ransom - Encinitas CA, US

International Classification:

G01N033/48

US Classification:

702021000

Abstract:

The present disclosure provides compositions and methods for high throughput Gain of Function (GOF) sorting to discover and develop novel and highly selective candidate drug molecules. High throughput GOF sorting includes: mutating a single residue in a receptor and/or ligand; measuring the affinity and functional activity of the resulting ligand target-ligand interaction; and carrying out multiple rounds of mutation and measurement to determine which residues provide key interaction points underlying the functional activity of a ligand target-ligand interaction. Further, the present disclosure provides methods and compositions for high throughput and high precision GOF sorting, such that large numbers of mutations can be generated and screened rapidly, and GOF compounds can be identified and isolated.

US Patent:

20050249642, Nov 10, 2005

Filed:

May 7, 2004

Appl. No.:

10/841239

Inventors:

Alex Okun - San Diego CA, US
John Ransom - Encinitas CA, US

Assignee:

Novasite Pharmaceuticals, Inc. - San Diego CA

International Classification:

B01L011/00

US Classification:

422103000

Abstract:

A system and method of mixing and injecting discrete sample mixtures into a flow cytometer or other sample analysis apparatus may generally comprise a sample injection guide coupling a liquid handling apparatus with a sample analysis apparatus and facilitating injection of discrete sample mixtures into a fluidic system of the apparatus.

US Patent:

20110065172, Mar 17, 2011

Filed:

Nov 18, 2010

Appl. No.:

12/949493

Inventors:

Alex Okun - San Diego CA, US
Teresa A. Bennett - Madrid, ES
Andrew Beemink - Fort Collins CO, US
David J. Sieg - Drexel Hill PA, US
John T. Ransom - Encinitas CA, US

Assignee:

Saryna Biotechnologies LLC - San Diego CA

International Classification:

C12M 1/34

US Classification:

4352871

Abstract:

A system and method of mixing and injecting discrete sample mixtures into a flow cytometer or other sample analysis apparatus may generally comprise a sample injection guide coupling a liquid handling apparatus with a sample analysis apparatus and facilitating injection of discrete sample mixtures into a fluidic system of the apparatus.