Claus Crone Fuglsang - Nivaa, DK Jens Sigurd Okkels - Frederiksberg, DK Dorte Aaby Petersen - Birkerod, DK Shamkant Anant Patkar - Lyngby, DK Marianne Thellersen - Frederiksberg, DK Allan Svendsen - Birkeroed, DK Kim Borch - Copenhagen, DK John C. Royer - Davis CA Titus Kretzschmar - Vaerloese, DK Torben Halkier - Birkeroed, DK Jesper Vind - Lyngby, DK Steen Troels Jorgensen - Alleroed, DK
Assignee:
Novozyme A/S - Bagsvaerd
International Classification:
C12N 920
US Classification:
435198, 435195, 435196, 435197
Abstract:
The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
Jeffrey R. Shuster - Davis CA John C. Royer - Davis CA
Assignee:
Novozymes Biotech, Inc. - Davis CA
International Classification:
C12N 114
US Classification:
4352541, 43525411, 435 691, 4351731
Abstract:
The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
Methods For Producing Heterologous Polypeptides In Trichothecene-Deficient Filamentous Fungal Mutant Cells
John C. Royer - Davis CA, US Lynne M. Christianson - Davis CA, US Gregory A. Gambetta - San Carlos CA, US Howard Brody - Davis CA, US Suzanne M. Otani - ELk Grove CA, US Wendy T. Yoder - Winters CA, US
The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells, isolated trichodiene synthases and nucleic acid sequences encoding the trichodiene synthases.
Methods For Improving Secondary Metabolite Production In Fungi
Robert Busby - Weymouth MA, US Brian Cali - Arlington MA, US Peter Hecht - Newton MA, US Doug Holtzman - Jamaica Plan MA, US Kevin Madden - Charlestown MA, US Mary Maxon - Somerville MA, US Todd Milne - Brookline MA, US Thea Norman - Belmont MA, US John Royer - Lexington MA, US Sofie Salama - Boston MA, US Amir Sherman - Boston MA, US Jeff Silva - Beverly MA, US Eric Summers - Brookline MA, US
Assignee:
Microbia, Inc. - Cambridge MA
International Classification:
C12P001/00 C12P001/02
US Classification:
435 41, 435 43, 43525411, 435471
Abstract:
The invention relates to the production of secondary metabolites by fungi. More particularly, the invention relates to improvement of production of commercially important secondary metabolites by fungi. The invention provides methods for improving secondary metabolite production in a fungus, comprising modulating the expression of a gene involved in regulation of secondary metabolite production.
The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
Non-Toxic Non-Toxigenic Non-Pathogenic Fusarium Expression System
The present invention relates non-toxic, non-toxigenic, non-pathogenic recombinant host cells of the section Discolor or a teleomorph or synonym thereof, comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter.
Shannon Roberts - Cambridge MA, US Amir Sherman - Jerusalem, IL Joshua Trueheart - Concord MA, US G. Todd Milne - Brookline MA, US John C. Royer - Lexington MA, US
The invention provides variant regulator proteins of secondary metabolite production and nucleic acids encoding said variant regulator proteins. In particular, the invention provides variant regulator molecules of the lovE protein.
Medical School University of Tennessee College of Medicine at Memphis Graduated: 1981
Languages:
English
Description:
Dr. Royer graduated from the University of Tennessee College of Medicine at Memphis in 1981. He works in Knoxville, TN and specializes in Diagnostic Radiology and Radiology. Dr. Royer is affiliated with Newport Medical Center and Turkey Creek Medical Center.
Resumes
Senior Principal Investigator, Team Leader New Products
Microbia
Senior Principal Investigator, Team Leader New Products
Novozymes Jan 1, 1993 - Jan 1, 1999
Senior Scientist
Microbia Jan 1, 1993 - Jan 1, 1999
Principal Investigator, Senior Principal Investigator, Team Leader New Product Development
Stanford University Sep 1, 1991 - May 1, 1993
Postdoctoral Fellow
University of Toronto May 1, 1988 - Aug 1, 1991
Postdoctoral Fellow
Education:
Stanford University 1991 - 1993
State University of New York College of Environmental Sciences and Forestry 1981 - 1988
Doctorates, Doctor of Philosophy, Biology
Suny College of Environmental
Master of Science, Doctorates, Masters, Doctor of Philosophy, Biology, Forestry
St. Lawrence University
Doctorates, Doctor of Philosophy
Spirit AeroSystems/Boeing - Manufacturing Process Analyst
Education:
Iowa State University - Industrial Engineering
About:
I was born in Iowa but have spent the last half of my life in Kansas. I enjoy drawing (my profile picture is a self portrait), reading, listening to music, swimming and making videos. People seem to...
Tagline:
Eschew obfuscation
Bragging Rights:
I once shook hands with Fred Grandy, "Gopher" from The Love Boat