Jonathan E Forman

US Patent:

6410675, Jun 25, 2002

Filed:

Apr 11, 2001

Appl. No.:

09/832490

Inventors:

Glenn McGall - Mountain View CA
Jonathan Eric Forman - San Jose CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C08G 7700

US Classification:

528 10, 528 25, 528 28, 528 33, 435 6, 536 221, 536 231, 536 243, 536 253, 536 2533, 422 681, 422 50

Abstract:

Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.

US Patent:

6429275, Aug 6, 2002

Filed:

Apr 11, 2001

Appl. No.:

09/832656

Inventors:

Glenn McGall - Mountain View CA
Jonathan Eric Forman - San Jose CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C08G 7700

US Classification:

528 10, 528 25, 528 28, 528 33, 435 6, 524588

Abstract:

Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.

US Patent:

6436647, Aug 20, 2002

Filed:

Mar 19, 2001

Appl. No.:

09/812706

Inventors:

Calvin F. Quate - Stanford CA
Mark O. Trulson - San Jose CA
Scott R. Manales - Santa Barbara CA
Jonathan E. Forman - San Jose CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 168

US Classification:

435 6, 435526

Abstract:

A method of using micromechanical devices as sensors for detecting chemical interactions between naturally occurring bio-polymers which are non-identical binding partners is provided. The method is useful whether the reactions occur through electrostatic forces or other forces. Induced stress, heat, or change in mass is detected where a binding partner is placed on a cantilever for possible reaction with an analyte molecules (i. e. , a non-identical binding partner). The method is particularly useful in determining DNA hybridization but may be useful in detecting interaction in any chemical assay.

US Patent:

6486286, Nov 26, 2002

Filed:

Oct 13, 1999

Appl. No.:

09/418044

Inventors:

Glenn McGall - Mountain View CA
Jonathan Eric Forman - San Jose CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C08G 7700

US Classification:

528 10, 528 25, 528 28, 528 33, 435 6, 536 221, 536 231, 536 253

Abstract:

Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.

US Patent:

6486287, Nov 26, 2002

Filed:

Apr 11, 2001

Appl. No.:

09/832489

Inventors:

Glenn McGall - Mountain View CA
Jonathan Eric Forman - San Jose CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C08G 7700

US Classification:

528 10, 528 25, 528 28, 528 33, 435 6, 536 221, 536 231, 536 253, 422 681, 422 50

Abstract:

Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.

US Patent:

6743882, Jun 1, 2004

Filed:

Oct 29, 2002

Appl. No.:

10/282979

Inventors:

Glenn McGall - Mountain View CA
Jonathan Eric Forman - San Jose CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C08G 7700

US Classification:

528 10, 528 25, 528 28, 528 33, 435 6, 524588

Abstract:

Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thio, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.

US Patent:

7098286, Aug 29, 2006

Filed:

May 28, 2004

Appl. No.:

10/857283

Inventors:

Glenn McGall - Mountain View CA, US
Jonathan Eric Forman - San Jose CA, US

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C08G 77/00
C08G 77/04

US Classification:

528 10, 528 25, 528 28, 528 33

Abstract:

Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.

US Patent:

7125947, Oct 24, 2006

Filed:

May 28, 2004

Appl. No.:

10/857329

Inventors:

Glenn McGall - Mountain View CA, US
Jonathan Eric Forman - San Jose CA, US

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C08G 77/00
C08G 77/04

US Classification:

528 10, 528 25, 528 28, 528 33

Abstract:

Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.