Joyce B Liu

US Patent:

20220389464, Dec 8, 2022

Filed:

Aug 5, 2022

Appl. No.:

17/817783

Inventors:

- Redwood City CA, US
Joyce Liu - Fremont CA, US
Nikki Dellas - San Carlos CA, US
Chinping Chng - Menlo Park CA, US
William Casey Hallows - San Francisco CA, US

International Classification:

C12P 7/42
C12P 13/22
C12N 15/63
A61K 9/00
A61K 31/122

Abstract:

The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity and enhanced acid stability, while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.

US Patent:

20210130855, May 6, 2021

Filed:

Jan 14, 2021

Appl. No.:

17/148834

Inventors:

- Redwood City CA, US
Joyce Liu - Fremont CA, US
Nikki Dellas - San Carlos CA, US
Chinping Chng - Menlo Park CA, US
William Casey Hallows - San Francisco CA, US

International Classification:

C12P 7/42
C12P 13/22
C12N 15/63
A61K 9/00
A61K 31/122

Abstract:

The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity and enhanced acid stability, while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.

US Patent:

20200032227, Jan 30, 2020

Filed:

Jul 17, 2019

Appl. No.:

16/514578

Inventors:

- Redwood City CA, US
- Hoffman Estates IL, US
Jack Liang - San Mateo CA, US
Joyce Liu - Fremont CA, US
Nikki Dellas - Mountain View CA, US
Melissa Ann Mayo - Foster City CA, US
David Entwistle - San Carlos CA, US

International Classification:

C12N 9/10
C12P 19/56
C07H 15/252

Abstract:

The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

US Patent:

20190376097, Dec 12, 2019

Filed:

Jun 10, 2019

Appl. No.:

16/436176

Inventors:

- Redwood City CA, US
Joyce Liu - Fremont CA, US
Nikki Dellas - Mountain View CA, US
Chinping Chng - Menlo Park CA, US
William Casey Hallows - San Francisco CA, US

International Classification:

C12P 7/42
C12P 13/22
C12N 15/63
A61K 31/122
A61K 9/00

Abstract:

The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity and enhanced acid stability, while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.

US Patent:

20180223264, Aug 9, 2018

Filed:

Feb 1, 2018

Appl. No.:

15/886094

Inventors:

- Redwood City CA, US
Stephanie Sue Galanie - Palo Alto CA, US
Nikki Dellas - Mountain View CA, US
Jack Liang - San Mateo CA, US
Joyce Liu - Fremont CA, US
David Entwistle - San Carlos CA, US
Courtney Dianne Moffett - San Francisco CA, US

International Classification:

C12N 9/10
C12N 9/88
C12P 19/56
C12P 19/18

Abstract:

The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.