Juan C Chavez

US Patent:

20140349871, Nov 27, 2014

Filed:

May 21, 2014

Appl. No.:

14/283902

Inventors:

- Seattle WA, US
Juan Chavez - Seattle WA, US
Chad Weisbrod - Seattle WA, US

International Classification:

G01N 33/68
G06F 19/16

US Classification:

506 9, 506 12, 530329, 703 11

Abstract:

Disclosed herein are methods for large-scale, high-throughput identification of protein-protein interactions and the topologies thereof under physiologically relevant conditions. In one aspect, the disclosure provides methods for identifying one or a plurality of interacting peptides within a biological system comprising obtaining a population of proteins cross-linked with a cleavable protein interaction reporter (PIR) cross-linker, cleaving the PIR crosslinker to produce released peptides and cleaved reporter ions, and analyzing the population of released peptides to identify interacting peptides. Also disclosed are methods for identifying candidate drug compounds, as well as methods of data processing and visualization of protein-protein interactions.