Paul F. Nealey - Madison WI Juan J. DePablo - Madison WI Francesco Cerrina - Madison WI Harun H. Solak - Brugg, CH XiaoMin Yang - Madison WI Richard D. Peters - Austin TX Qiang Wang - Madison WI
Copolymer structures are formed by exposing a substrate with an imaging layer thereon to two or more beams of selected wavelengths to form interference patterns at the imaging layer to change the wettability of the imaging layer in accordance with the interference patterns. A layer of a selected block copolymer is deposited onto the exposed imaging layer and annealed to separate the components of the copolymer in accordance with the pattern of wettability and to replicate the pattern of the imaging layer in the copolymer layer. Stripes or isolated regions of the separated components may be formed with periodic dimensions in the range of 100 nm or less.
Preservation And Storage Medium For Biological Materials
Juan DePablo - Madison WI, US Danforth Miller - Madison WI, US Paul Conrad - Madison WI, US Horacio Corti - Lanus Buenos Aires, AR
Assignee:
Wisconsin Alumni Research Foundation - Madison MI
International Classification:
A01N001/00 A01N001/02 A61K031/7012
US Classification:
435 11, 435 2, 514 53
Abstract:
A protectant mixture for use in preserving biological materials comprising (1) at least one polyhydroxy compound, where the total amount of polyhydroxy compound in the mixture is from about 5% to about 60% by weight of the mixture where the mixture is an aqueous solution and is from about 10% to about 95% where the mixture is in solid form, and (2) phosphate ions, where the total amount of phosphate ions in the mixture is such that the molar ratio of phosphate ions to hydroxy groups in the polyhydroxy compound is from about 0. 025 to about 0. 625; a preservation medium comprising (1) a biological material, (2) at least one polyhydroxy compound, where the total amount of polyhydroxy compound in the medium is from about 5% to about 60% by weight of the medium, and (3) phosphate ions, where the total amount of phosphate ions in the mixture is such that the molar ratio of phosphate ions to hydroxy groups in the polyhydroxy compound is from about 0. 025 to about 0. 625; methods of preserving the preservation medium; and the resulting preserved biological material composition.
Guided Self-Assembly Of Block Copolymer Films On Interferometrically Nanopatterned Substrates
Paul F. Nealey - Madison WI, US Juan J. DePablo - Madison WI, US Francesco Cerrina - Madison WI, US Harun H. Solak - Brugg, CH XiaoMin Yang - Madison WI, US Richard D. Peters - Austin TX, US Qiang Wang - Madison WI, US
Copolymer structures are formed by exposing a substrate with an imaging layer thereon to two or more beams of selected wavelengths to form interference patterns at the imaging layer to change the wettability of the imaging layer in accordance with the interference patterns. A layer of a selected block copolymer is deposited onto the exposed imaging layer and annealed to separate the components of the copolymer in accordance with the pattern of wettability and to replicate the pattern of the imaging layer in the copolymer layer. Stripes or isolated regions of the separated components may be formed with periodic dimensions in the range of 100 nm or less.
Juan DePablo - Madison WI, US Ying Nie - Madison WI, US Sean P. Palecek - Madison WI, US
Assignee:
Wisconsin Alumni Research Foundation - Madison WI
International Classification:
C12N 5/06 A61K 31/702
US Classification:
435374, 424532, 435 2, 514 53
Abstract:
A protectant mixture for use in preserving biological materials comprising at least one polyhydroxy compound, and phosphate ions; a preservation medium comprising a biological material, at least one polyhydroxy compound, and phosphate ions; methods of preserving the preservation medium; and the resulting preserved biological material composition.
Nicholas L. Abbott - Madison WI, US Jeffrey C. Mohr - Madison WI, US Christopher J. Murphy - Madison WI, US Elise Huang - Fitchburg WI, US Juan J. DePablo - Madison WI, US Nathan A. Lockwood - Minneapolis MN, US Sean P. Palecek - Madison WI, US
The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e. g. , reorganization) of extracellular matrix components by living cells.
Nicholas L. Abbott - Madison WI, US Jeffrey C. Mohr - Madison WI, US Christopher J. Murphy - Madison WI, US Elise Huang - Fitchburg WI, US Juan J. DePablo - Madison WI, US Nathan A. Lockwood - Minneapolis MN, US Sean P. Palecek - Madison WI, US
The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e. g. , reorganization) of extracellular matrix components by living cells.
Cryopreservation Of Human Embryonic Stem Cells In Microwells
Sean P. Palecek - Madison WI, US Juan J. DePablo - Madison WI, US Jeffrey C. Mohr - Madison WI, US Lin Ji - Madison WI, US
International Classification:
C12N 5/06 C12M 1/00
US Classification:
435374, 4353071
Abstract:
The present invention relates to methods and structures for preparing stem cells for use in cryopreservation methods. Stem cell colonies are provided between first and second matrix portions and are exposed to a carbohydrate-containing cryoprotecting medium and a freezing medium. The methods of the invention yield cryopreserved cells that maintain cell viability and exhibit limited cell differentiation after freezing and thawing, facilitating storage, shipping and handling of embryonic stem cell stocks and lines for research and therapeutics.
Nanoparticle Modified Lubricants And Waxes With Enhanced Properties
The present invention provides compositions and products, such as waxes and lubricants, comprising a plurality of nanoparticles dispersed in a continuous phase comprising a vegetable oil derived material, such as one or more vegetable oils or a synthetic product derived from one or more vegetable oils. Incorporation of nanoparticles in the present compositions is beneficial for providing mechanical, thermal and/or chemical properties useful for a selected product or product application. In some compositions of the present invention, for example, incorporation of the nanoparticle component provides compositions derived from one or more vegetable oils exhibiting enhanced mechanical stability, hardness, viscosity, thermal stability and mechanical strength.
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