Julie A Moore

US Patent:

20030129716, Jul 10, 2003

Filed:

Dec 6, 2002

Appl. No.:

10/313893

Inventors:

Charles Ragsdale - Concord CA, US
Julie Moore - Sausalito CA, US
William Heiser - Benicia CA, US
Tony Chiu - San Leandro CA, US
Keith Kaste - El Cerrito CA, US

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

C12N013/00

US Classification:

435/173600

Abstract:

Systems, methods and algorithms for automatically performing optimization of an electroporation system. A system according to the present invention typically includes a cuvette holding assembly configured to hold a plurality of electroporation cuvettes, wherein each cuvette includes a first and second electrode, and a shocking chamber configured to hold the cuvette holding assembly, the chamber having a commutator assembly configured to provide an electrical contact to the first electrode of each of the plurality of cuvettes in turn. The system also typically includes a control system communicably coupled to the shocking chamber, wherein the control system controls the commutator to automatically contact the first electrode of each cuvette in an order and to provide a potential across the cuvette electrodes when contact is made.