Jun M Lee

US Patent:

6495350, Dec 17, 2002

Filed:

Dec 23, 1999

Appl. No.:

09/472066

Inventors:

Jun E. Lee - North Potomac MD
Ayoub Rashtchian - Gaithersburg MD

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 168

US Classification:

435 912, 435 6, 435 71, 435 911, 435 9151, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

Abstract:

The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

US Patent:

6767724, Jul 27, 2004

Filed:

Aug 21, 2002

Appl. No.:

10/224334

Inventors:

Jun E. Lee - North Potomac MD
Ayoub Rashtchian - Gaithersburg MD

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12P 1934

US Classification:

435 912, 435 6, 435 71, 435 911, 435180, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

Abstract:

The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

US Patent:

7883871, Feb 8, 2011

Filed:

Jun 7, 2004

Appl. No.:

10/861469

Inventors:

Jun E. Lee - North Potomac MD, US
Ayoub Rashtchian - Gaithersburg MD, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12P 19/34
C07H 21/04

US Classification:

435 9121, 435 912, 536 231, 536 232

Abstract:

The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

US Patent:

20040209276, Oct 21, 2004

Filed:

Sep 15, 2003

Appl. No.:

10/661819

Inventors:

Michael Smith - Rockville MD, US
Robert Potter - San Marcos CA, US
Gulshan Dhariwal - Potomac MD, US
Gary Gerard - Frederick MD, US
Kim Rosenthal - Laytonsville MD, US
Jun Lee - San Diego CA, US

International Classification:

C12Q001/68
C07H021/04
C12P019/34
C12N009/22
C12N015/74

US Classification:

435/006000, 435/069100, 435/091200, 435/199000, 435/252300, 435/320100, 536/023200

Abstract:

The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

US Patent:

20070141592, Jun 21, 2007

Filed:

Jul 25, 2006

Appl. No.:

11/492194

Inventors:

Michael Smith - Rockville MD, US
Robert Potter - San Marcos CA, US
Gulshan Dhariwal - Potomac MD, US
Gary Gerard - Frederick MD, US
Kim Rosenthal - Laytonsille MD, US
Jun Lee - San Diego CA, US

International Classification:

C12Q 1/68
C07H 21/04
C12P 21/06
C12N 9/22
C12N 15/86

US Classification:

435006000, 435069100, 435199000, 435456000, 435325000, 536023200

Abstract:

The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

US Patent:

20120021464, Jan 26, 2012

Filed:

Dec 28, 2010

Appl. No.:

12/979899

Inventors:

Michael D. SMITH - Rockville MD, US
Robert Jason POTTER - San Marcos CA, US
Gulshan DHARIWAL - Potomac MD, US
Gary F. GERARD - Frederick MD, US
Kim ROSENTHAL - Laytonsville MD, US
Jun E. LEE - San Diego CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12P 19/34
C12N 9/12

US Classification:

435 9151, 435194

Abstract:

The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

US Patent:

59035439, May 11, 1999

Filed:

May 21, 1996

Appl. No.:

8/652020

Inventors:

Jun Guy Lee - Gaithersburg MD
Young I. L. Kim - Gaithersburg MD
Sung Min Song - Gaithersburg MD

Assignee:

Samsung Electronics Co., Ltd. - Suwon

International Classification:

H04J3/06

US Classification:

370216

Abstract:

In a clock system having active and standby clocks for synchronizing cell data flow in a data system, and for preventing cell data loss during active to standby clock switching, a prevent circuit maintains the active clock cycle signal during the active/standby clock switching to ensure the proper setup time before the active clock is disabled. The prior standby clock will become active during the maintenance period, thereby providing the proper setup time before and after switching, permitting system redundancy without loss of data.