Jun K Lee

US Patent:

20040126789, Jul 1, 2004

Filed:

Sep 5, 2003

Appl. No.:

10/655579

Inventors:

Kyusung Park - San Marcos CA, US
Jun Lee - San Diego CA, US

Assignee:

Invitrogen Corporation

International Classification:

C12Q001/68
C12P019/34
C07K016/40

US Classification:

435/006000, 530/388260, 435/091200

Abstract:

Disclosed are compositions preferably for use in nucleic acid synthesis that include one or more anti-reverse transcriptase (RT) antibodies and/or one or more anti-DNA polymerase (DNAP) antibodies and/or single strand binding proteins (SSBs). Some of the disclosed compositions include one or more anti-DNAP antibodies and/or one or more anti-RT antibodies and one or more SSBs. Other disclosed compositions include two or more SSBs. The disclosed nucleic acid synthesis compositions also can include one or more DNAPs, one or more RTs, one or more nucleotides, one or more primers, and/or one or more templates. Also disclosed are methods for using such compositions in nucleic acid synthesis, amplification and sequencing, where various combinations of anti-RT antibodies, anti-DNAP antibodies and/or SSBs can improve the yield and/or homogeneity of primer extension products.

US Patent:

20060172312, Aug 3, 2006

Filed:

Jan 28, 2005

Appl. No.:

11/044620

Inventors:

John Bishop - Carlsbad CA, US
Jun Lee - San Diego CA, US

International Classification:

C12Q 1/68
C12N 9/00
C07K 16/46

US Classification:

435006000, 530391100, 435188500

Abstract:

The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a molecule or complex of molecules that binds to a PBM (i.e., a PBM-binding molecule). The combination of the PBM and PBM-binding molecule/complex substantially inhibits polymerase activity. The disclosed multi-component inhibitors are useful for DNA sequencing, nucleic acid amplification, cloning and synthesis, and the like.

US Patent:

20060216735, Sep 28, 2006

Filed:

Jan 30, 2006

Appl. No.:

11/341870

Inventors:

John Bishop - Carlsbad CA, US
Jun Lee - San Diego CA, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 1/68
C12P 19/34
C12N 9/22

US Classification:

435006000, 435091200, 435199000

Abstract:

The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a molecule or complex of molecules that binds to a PBM (i.e., a PBM-binding molecule). The combination of the PBM and PBM-binding molecule/complex substantially inhibits polymerase activity.

US Patent:

20060292578, Dec 28, 2006

Filed:

Jun 28, 2005

Appl. No.:

11/170762

Inventors:

Weidong Zheng - Carlsbad CA, US
Jun Lee - San Diego CA, US
Robert Potter - San Marcos CA, US
David Mandelman - Carlsbad CA, US

International Classification:

C12Q 1/68
C07H 21/04
C12P 21/06
C12P 19/34
C12N 9/22
C12N 15/74
C12N 1/21

US Classification:

435006000, 435069100, 435091200, 435199000, 435252300, 435471000, 536023200

Abstract:

A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis,

US Patent:

20070178491, Aug 2, 2007

Filed:

Nov 9, 2006

Appl. No.:

11/558294

Inventors:

Kyusung Park - Vista CA, US
Jun Lee - San Diego CA, US

Assignee:

INVITROGEN CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68
C12P 19/34
C07K 16/40

US Classification:

435006000, 435091200, 530388260

Abstract:

Disclosed are compositions preferably for use in nucleic acid synthesis that include one or more anti-reverse transcriptase (RT) antibodies and/or one or more anti-DNA polymerase (DNAP) antibodies and/or single strand binding proteins (SSBs). Some of the disclosed compositions include one or more anti-DNAP antibodies and/or one or more anti-RT antibodies and one or more SSBs. Other disclosed compositions include two or more SSBs. The disclosed nucleic acid synthesis compositions also can include one or more DNAPs, one or more RTs, one or more nucleotides, one or more primers, and/or one or more templates. Also disclosed are methods for using such compositions in nucleic acid synthesis, amplification and sequencing, where various combinations of anti-RT antibodies, anti-DNAP antibodies and/or SSBs can improve the yield and/or homogeneity of primer extension products.

US Patent:

20080254525, Oct 16, 2008

Filed:

Mar 20, 2008

Appl. No.:

12/052682

Inventors:

Weidong Zheng - Carlsbad CA, US
Jun E. Lee - San Diego CA, US
Robert Jason Potter - San Marcos CA, US
David Mandelman - Carlsbad CA, US

Assignee:

INVITROGEN CORPORATION - Carlsbad CA

International Classification:

C12N 9/10

US Classification:

435193

Abstract:

A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis,

US Patent:

20100068767, Mar 18, 2010

Filed:

Aug 13, 2009

Appl. No.:

12/541119

Inventors:

John D. Bishop - Carlsbad CA, US
Jun E. Lee - San Diego CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12P 19/34
C12N 9/10
C12N 9/08

US Classification:

435 915, 435193, 435192

Abstract:

The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a molecule or complex of molecules that binds to a PBM (i.e., a PBM-binding molecule). The combination of the PBM and PBM-binding molecule/complex substantially inhibits polymerase activity. The disclosed multi-component inhibitors are useful for DNA sequencing, nucleic acid amplification, cloning and synthesis, and the like.

US Patent:

20100209975, Aug 19, 2010

Filed:

Dec 11, 2009

Appl. No.:

12/636655

Inventors:

John D. BISHOP - Carlsbad CA, US
Jun E. LEE - San Diego CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12P 19/34
C12N 9/10
C12N 9/99

US Classification:

435 915, 435193, 435184

Abstract:

The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a molecule or complex of molecules that binds to a PBM (i.e., a PBM-binding molecule). The combination of the PBM and PBM-binding molecule/complex substantially inhibits polymerase activity.