Jun Jun Lee

US Patent:

8372604, Feb 12, 2013

Filed:

Dec 29, 2010

Appl. No.:

12/981240

Inventors:

Jun Lee - San Diego CA, US
Ayoub Rashtchian - Gaithersburg MD, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12P 19/34

US Classification:

435 912, 435 9121

Abstract:

The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

US Patent:

20040126789, Jul 1, 2004

Filed:

Sep 5, 2003

Appl. No.:

10/655579

Inventors:

Kyusung Park - San Marcos CA, US
Jun Lee - San Diego CA, US

Assignee:

Invitrogen Corporation

International Classification:

C12Q001/68
C12P019/34
C07K016/40

US Classification:

435/006000, 530/388260, 435/091200

Abstract:

Disclosed are compositions preferably for use in nucleic acid synthesis that include one or more anti-reverse transcriptase (RT) antibodies and/or one or more anti-DNA polymerase (DNAP) antibodies and/or single strand binding proteins (SSBs). Some of the disclosed compositions include one or more anti-DNAP antibodies and/or one or more anti-RT antibodies and one or more SSBs. Other disclosed compositions include two or more SSBs. The disclosed nucleic acid synthesis compositions also can include one or more DNAPs, one or more RTs, one or more nucleotides, one or more primers, and/or one or more templates. Also disclosed are methods for using such compositions in nucleic acid synthesis, amplification and sequencing, where various combinations of anti-RT antibodies, anti-DNAP antibodies and/or SSBs can improve the yield and/or homogeneity of primer extension products.

US Patent:

20040209276, Oct 21, 2004

Filed:

Sep 15, 2003

Appl. No.:

10/661819

Inventors:

Michael Smith - Rockville MD, US
Robert Potter - San Marcos CA, US
Gulshan Dhariwal - Potomac MD, US
Gary Gerard - Frederick MD, US
Kim Rosenthal - Laytonsville MD, US
Jun Lee - San Diego CA, US

International Classification:

C12Q001/68
C07H021/04
C12P019/34
C12N009/22
C12N015/74

US Classification:

435/006000, 435/069100, 435/091200, 435/199000, 435/252300, 435/320100, 536/023200

Abstract:

The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

US Patent:

20040265870, Dec 30, 2004

Filed:

Apr 9, 2004

Appl. No.:

10/821128

Inventors:

Jun Lee - San Diego CA, US
Weidong Zheng - Carlsbad CA, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q001/68
C07H021/02
C12P019/34

US Classification:

435/006000, 435/091200, 536/025300

Abstract:

The present invention is generally related to composition, kits and methods for synthesizing nucleic acid molecules and particularly for synthesizing labeled nucleic acid molecules. Specifically, the invention relates to methods, kits and compositions for synthesizing indirectly labeled nucleic acid molecules. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection, diagnostics, and array analysis.

US Patent:

20060105348, May 18, 2006

Filed:

Nov 15, 2004

Appl. No.:

10/987453

Inventors:

Jun Lee - San Diego CA, US
Robert Potter - San Marcos CA, US
Jonathan Nery - Escondido CA, US

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435006000, 536025320

Abstract:

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

US Patent:

20060172312, Aug 3, 2006

Filed:

Jan 28, 2005

Appl. No.:

11/044620

Inventors:

John Bishop - Carlsbad CA, US
Jun Lee - San Diego CA, US

International Classification:

C12Q 1/68
C12N 9/00
C07K 16/46

US Classification:

435006000, 530391100, 435188500

Abstract:

The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a molecule or complex of molecules that binds to a PBM (i.e., a PBM-binding molecule). The combination of the PBM and PBM-binding molecule/complex substantially inhibits polymerase activity. The disclosed multi-component inhibitors are useful for DNA sequencing, nucleic acid amplification, cloning and synthesis, and the like.

US Patent:

20060216735, Sep 28, 2006

Filed:

Jan 30, 2006

Appl. No.:

11/341870

Inventors:

John Bishop - Carlsbad CA, US
Jun Lee - San Diego CA, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 1/68
C12P 19/34
C12N 9/22

US Classification:

435006000, 435091200, 435199000

Abstract:

The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a molecule or complex of molecules that binds to a PBM (i.e., a PBM-binding molecule). The combination of the PBM and PBM-binding molecule/complex substantially inhibits polymerase activity.

US Patent:

20060275792, Dec 7, 2006

Filed:

Feb 28, 2006

Appl. No.:

11/365383

Inventors:

Jun Lee - San Diego CA, US
Robert Potter - San Marcos CA, US

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

Disclosed are methods and materials for enhancing the efficiency, specificity and/or specificity of nucleic acid amplification reactions (e.g., PCR amplification reactions). The disclosed inventions involve the use of nonspecific double-stranded DNA binding proteins in amplification reactions. The methods described herein are useful in a wide variety of molecular biology applications including allele specific PCR.