Jun S Song

US Patent:

20080131940, Jun 5, 2008

Filed:

Jun 22, 2005

Appl. No.:

11/570423

Inventors:

Robert Chiu - Tustin CA, US
Jun Song - Los Angeles CA, US

International Classification:

C12P 19/34
C07H 21/02
C12N 15/63
C12N 5/00
C12N 15/87

US Classification:

435 913, 536 231, 4353201, 435325, 435463

Abstract:

The present invention provides nucleic acids that include a nucleotide sequence that encodes an siRNA, which nucleotide sequence is operably linked to a target cell-specific promoter RNA polymerase II promoter. The present invention further provides vectors, including expression vectors, which include a subject nucleic acid; and host cells that harbor a subject nucleic acid or a subject expression vector. The present invention further provides methods of modulating (e.g., reducing) expression of a gene in a target cell-specific manner, the methods generally involving introducing into a cell a subject expression vector.