Abstract:
A system is provided for reducing non-specific binding of an enzyme to lignin to enhance an enzymatic processing of a lignocellulosic material. The enhancements provide economic and process advantages to any process that converts a lignocellulosic biomass into a product using an enzyme. Systems are provided comprising a reaction vessel; a lignocellulosic feedstock comprising a component selected from the group consisting of a hardwood, a softwood, or a non-wood material; an enzyme component including a cellulase, a hemicellulase, or a combination thereof; and, water. The reaction vessel can contain a combination of the lignocellulosic feedstock, the water, and the enzyme component at a pH ranging from about 5.2 to about 6.2; and, the lignocellulosic feedstock can be saccharified in the reaction vessel. Moreover, the systems can include a lignosulfonate, with or without a pH of about 5.2 to about 6.2, to also reduce non-specific binding and enhance enzymatic activity.