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Us Patents
Methods And Compositions For Preparation Of Nucleic Acids
- Basel, CH Wenling Wang - Durham NC, US Karen Elizabeth Moore - Durham NC, US Kimberly Ann White - Durham NC, US
Assignee:
SYNGENTA PARTICIPATIONS AG - Basel
International Classification:
C12Q 1/68
US Classification:
435 612
Abstract:
A method for isolating genomic DNA (gDNA) from a biological material. In some embodiments, the method includes (a) contacting a sample that contains gDNA with a solution of hydroxide and a detergent under conditions and for a time sufficient to degrade a cell wall, a cell membrane, a nuclear membrane, a nucleoprotein, or combinations thereof and/or to denature the gDNA; (b) mixing into the solution resulting from step (a) a solution characterized by high salt and sufficient buffering capacity to reduce the pH of the solution to less than 10, thereby producing a neutralized solution; (c) centrifuging the sample at a speed and length of time sufficient to clarify the preparation; and (d) removing insoluble material from the neutralized and clarified preparation, whereby a solution of gDNA is produced. Also provided are method for performing a quantitative polymerase chain reaction (qPCR) of a gDNA sample, methods for performing genotyping or other molecular marker analysis of a gDNA sample, and methods for determining a haplotype of a cell.
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First filed in April 2019 over back pay, lost benefits and more by Disney staffers LaRonda Rasmussen and Karen Moore and heading towards a May 2025 trial, the suit accused the Magic Kingdom of not being so kind with its cash based on gender as opposed to performance. At its core, the suit claimed Di
Date: Nov 25, 2024
Category: Business
Source: Google
Disney’s Bob Iger Defends $66M Pay Package But Says “Subject Of Income Inequality Is Very, Very Real” – Vanity Fair Summit
Longtime Walt Disney Studios employees LaRonda Rasmussen and Karen Moore instigated the suit on April 3 in a move for back pay, lost benefits and other compensation. They were joined by eight other women last month in an amended complaint that Disney now wants to see shredded just like the attorne
Date: Oct 22, 2019
Category: Entertainment
Source: Google
Breaking Bad Turns 10: An Oral History of the Pilot
Karen Moore (line producer): [My agent] said, "Oh, the script is written by our client Vince Gilligan, and he's also going to direct it." I said, "Great. What else has he directed?" And there was this long pause and they went, "Ummm, an episode, maybe two episodes of X-Files."
"We are experiencing a slow period of time with donations, and our collections are very low going deep into the summer vacation season," said Karen Moore, account manager with the Central Jersey Blood Center. "Giving out the gas cards as a thank you to our donors helps."
Elionna Maria Johnson-Moore, infant daughter of Karen Moore and Justin Johnson, passed away on Friday, Feb. 15, 2013, at the Children's Hospital in Minneapolis, Minn.