Katherine A Fisher

US Patent:

20210254124, Aug 19, 2021

Filed:

Feb 10, 2021

Appl. No.:

17/172726

Inventors:

Leah Wickenberg - Reno NV, US
Avneet K. Chhabra - Sparks NV, US
Katherine E. Fisher - Reno NV, US
William F. McCoy - Reno NV, US

International Classification:

C12Q 1/04

Abstract:

To determine the presence of in an environment, a sample from the environment can be plated onto a growth medium that is selective for The agar based growth medium can include a high concentration of crystal violet, in excess of 0.5 μg/ml. The process may be made further selective for by treating the sample with sodium dodecyl sulfate containing glycine hydrochloride for at least 4 minutes at room temperature, prior to plating. colonies will generally appear white while other colonies will generally appear stained purple or another color.

US Patent:

20200208200, Jul 2, 2020

Filed:

Dec 29, 2019

Appl. No.:

16/729422

Inventors:

Leah Wickenberg - Reno NV, US
Katherine Fisher - Reno NV, US
William F. McCoy - Reno NV, US

International Classification:

C12Q 1/689
C12Q 1/6806
C12Q 1/686
B01D 71/50
B01D 69/02

Abstract:

A process for detecting viable waterborne pathogens in a water sample, includes enriching a concentrate of bacteria from the sample and taking a first DNA extract at time Tand a second DNA extract at time Tafter an incubation period. Real-time polymerase chain reaction performed on the DNA extracts yields respective cycle threshold values Ct. The change in Ct provides an indication of the targeted pathogen in the sample. An order of magnitude quantification of the sample can also be performed using a serial dilution technique on the TDNA extract. The process has particular application for detecting , including viable but not culturable cells.