Katherine M Pfeiffer

US Patent:

20220403350, Dec 22, 2022

Filed:

Jun 10, 2022

Appl. No.:

17/837497

Inventors:

- Pleasanton CA, US
Derek Hunter Vallejo - Pleasanton CA, US
Carolina Cuellar - Pleasanton CA, US
Katherine Pfeiffer - Pleasanton CA, US

Assignee:

10X Genomics, Inc. - Pleasanton CA

International Classification:

C12N 9/12

Abstract:

The application provides compositions including engineered reverse transcriptases with at least one altered reverse-transcriptase related activity. The engineered reverse transcriptases or reverse transcription enzymes unexpectedly exhibit one or more altered reverse transcriptase related activities such as but not limited to altered template switching efficiency, altered transcription efficiency or both.

US Patent:

20220276229, Sep 1, 2022

Filed:

Feb 24, 2022

Appl. No.:

17/680209

Inventors:

- Pleasanton CA, US
Eswar Prasad Ramachandran Iyer - Sunnyvale CA, US
Andrew Kohlway - Pleasanton CA, US
Luigi Jhon Alvarado Martinez - Walnut Creek CA, US
Katherine Pfeiffer - Oakland CA, US
Andrew D. Price - Hayward CA, US

International Classification:

G01N 33/532
C07K 14/74
C12N 15/85
G01N 33/543
G01N 33/548
C12N 15/10
C12Q 1/6804
C12Q 1/6806
G01N 33/50
G01N 33/53
G01N 33/58
C12Q 1/6818
C12Q 1/6827
C12Q 1/6881
C12N 15/11
G01N 33/569

Abstract:

The present disclosure provides methods of processing or analyzing a sample. A method for processing a sample may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), barcoding the probe-nucleic acid molecule complex, and performing extension, denaturation, and amplification processes. A method for processing a sample may comprise hybridizing first and second probes to adjacent or non-adjacent target regions of a nucleic acid molecule (e.g., an RNA molecule), linking the first and second probes to provide a probe-linked nucleic acid molecule, and barcoding the probe-linked nucleic acid molecule. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. One or more processes of the methods described herein may be performed on a cell, such as a permeabilized cell.

US Patent:

20220228220, Jul 21, 2022

Filed:

Aug 31, 2021

Appl. No.:

17/462712

Inventors:

- Pleasanton CA, US
Michael Ybarra Lucero - Pleasanton CA, US
Elliott Meer - Pleasanton CA, US
Tarjei Sigurd Mikkelsen - Pleasanton CA, US
Katherine Pfeiffer - Pleasanton CA, US
Sarah Taylor - Pleasanton CA, US
Niranjan Srinivas - Pleasanton CA, US

International Classification:

C12Q 1/6886
C12N 15/10
G16B 30/00
G16B 50/00
C12Q 1/6881
C12Q 1/6804

Abstract:

The present disclosure provides methods, systems, and compositions for parallel processing of nucleic acid samples. Methods and systems of the present disclosure comprise the use of sample-specific barcode sequences, which facilitate the multiplexing of samples, detection of discrete cell populations within a pooled population, and detection of partitions comprising more than one cell.

US Patent:

20220195420, Jun 23, 2022

Filed:

Nov 2, 2021

Appl. No.:

17/517282

Inventors:

- Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Pleasanton CA, US
Bill Kengli Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Pranav Patel - Fremont CA, US
Katherine Pfeiffer - Berkeley CA, US
Andrew D. Price - Hayward CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US

International Classification:

C12N 15/10
C12Q 1/6806
C12Q 1/6869

Abstract:

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

US Patent:

20220195422, Jun 23, 2022

Filed:

Mar 9, 2022

Appl. No.:

17/690628

Inventors:

- Pleasanton CA, US
Marlon Stoeckius - Stockholm, SE
Katherine Pfeiffer - Pleasanton CA, US

Assignee:

10x Genomics, Inc. - Pleasanton CA

International Classification:

C12N 15/10
C12Q 1/6837
C12Q 1/6874

Abstract:

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

US Patent:

20210372998, Dec 2, 2021

Filed:

Mar 22, 2021

Appl. No.:

17/209045

Inventors:

- Pleasanton CA, US
Jason Bell - Palo Alto CA, US
Zachary Bent - Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Livermore CA, US
Bill Kengli Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Geoffrey McDermott - Livermore CA, US
Elliott Meer - Pleasanton CA, US
Francesca Meschi - Menlo Park CA, US
Tarjei Sigurd Mikkelsen - Dublin CA, US
Christopher Joachim O'Keeffe - Pleasanton CA, US
Katherine Pfeiffer - Pleasanton CA, US
Andrew D. Price - Hayward CA, US
Paul Ryvkin - San Jose CA, US
Sarah Taylor - Fremont CA, US
Jessica Michele Terry - Pleasanton CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US
Xinying Zheng - San Jose CA, US
Solongo Batjargal Ziraldo - San Ramon CA, US
Eswar Prasad Ramachandran Iyer - Sunnyvale CA, US
Luigi Jhon Alvarado Martinez - Pleasanton CA, US

International Classification:

G01N 33/532
C07K 14/74
C12N 15/85
G01N 33/543
G01N 33/548
C12N 15/10
C12Q 1/6804
C12Q 1/6806
G01N 33/50
G01N 33/53
G01N 33/58
C12Q 1/6818
C12Q 1/6827
C12Q 1/6881
C12N 15/11
G01N 33/569

Abstract:

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.

US Patent:

20210222158, Jul 22, 2021

Filed:

Mar 22, 2021

Appl. No.:

17/208369

Inventors:

- Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Pleasanton CA, US
Bill Kengli Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Pranav Patel - Fremont CA, US
Katherine Pfeiffer - Berkeley CA, US
Andrew D. Price - Hayward CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US

International Classification:

C12N 15/10
C12Q 1/6806
C12Q 1/6869

Abstract:

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

US Patent:

20210130892, May 6, 2021

Filed:

Oct 29, 2020

Appl. No.:

17/084481

Inventors:

- Pleasanton CA, US
Zachary Bent - Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Vijay Kumar Sreenivasa Gopalan - Pleasanton CA, US
Josephine Harada - San Francisco CA, US
Christopher Hindson - Livermore CA, US
Michael Ybarra Lucero - South San Francisco CA, US
Geoffrey McDermott - Livermore CA, US
Elliott Meer - Pleasanton CA, US
Tarjei Sigurd Mikkelsen - Dublin CA, US
Christopher Joachim O'Keeffe - Pleasanton CA, US
Katherine Pfeiffer - Berkeley CA, US
Andrew D. Price - Hayward CA, US
Paul Ryvkin - San Jose CA, US
Serge Saxonov - Oakland CA, US
John R. Stuelpnagel - Santa Barbara CA, US
Jessica Michele Terry - Pleasanton CA, US
Tobias Daniel Wheeler - Alameda CA, US
Indira Wu - San Carlos CA, US
Solongo Batjargal Ziraldo - San Ramon CA, US
Stephane Claude Boutet - Burlingame CA, US
Sarah Taylor - Pleasanton CA, US
Niranjan Srinivas - Dublin CA, US

International Classification:

C12Q 1/6874
C12Q 1/683
C12Q 1/6806

Abstract:

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.