Kathleen J Doane

US Patent:

7732219, Jun 8, 2010

Filed:

Sep 10, 2007

Appl. No.:

11/852662

Inventors:

Christopher J. Woolverton - Kent OH, US
Gary D. Niehaus - Kent OH, US
Kathleen J. Doane - Ravenna OH, US
Oleg D. Lavrentovich - Kent OH, US
Steven P. Schmidt - Akron OH, US
Steven A. Signs - Hudson OH, US

Assignee:

Kent State University - Kent OH

International Classification:

G01N 33/553
G01N 33/53

US Classification:

436525, 436518, 435 71, 435 5, 435 79, 435 72, 435 691, 349123, 349124, 349130, 349137, 536 234, 536 235

Abstract:

A system for the detection of ligands comprising at least one receptor and an amplification mechanism coupled to the receptor wherein an amplified signal is produced as a result of receptor binding a ligand. Examples of suitable amplification mechanisms include antibody-embedded liquid crystalline materials; use of alpha-2-macroglobulin to encage an enzyme, whereby the enzyme is separated from its substrate by an receptor; and a receptor engineered to inhibit the active of site of an enzyme only in the absence of a ligand. Also provided are methods for the automatic detection of ligands.

US Patent:

7927827, Apr 19, 2011

Filed:

May 21, 2007

Appl. No.:

11/751065

Inventors:

Gary D. Niehaus - Kent OH, US
Christopher J. Woolverton - Kent OH, US
Kathleen J. Doane - Ravenna OH, US
Steven P. Schmidt - Akron OH, US
Steven A. Signs - Hudson OH, US

Assignee:

Kent State University - Kent OH

International Classification:

G01N 33/53
C12Q 1/00

US Classification:

435 79, 435 78, 435174, 435182

Abstract:

A system for the detection of ligands comprising at least one receptor and an amplification mechanism coupled to the receptor wherein an amplified signal is produced as a result of receptor binding a ligand. Examples of suitable amplification mechanisms include antibody-embedded liquid crystalline materials; use of alpha-2-macroglobulin to encage an enzyme, whereby the enzyme is separated from its substrate by an receptor; and a receptor engineered to inhibit the active of site of an enzyme only in the absence of a ligand. Also provided are methods for the automatic detection of ligands.

US Patent:

7267957, Sep 11, 2007

Filed:

Apr 26, 2002

Appl. No.:

10/133610

Inventors:

Christopher J Woolverton - Kent OH, US
Gary D Niehaus - Kent OH, US
Kathleen J Doane - Ravenna OH, US
Oleg D Lavrentovich - Kent OH, US
Steven P Schmidt - Akron OH, US
Steven A Signs - Hudson OH, US

Assignee:

Kent State University - Kent OH

International Classification:

G01N 33/53
C12P 21/06

US Classification:

435 71, 435 79, 435 72, 435 691, 435 5, 349123, 349124, 349130, 349137, 530350, 536 234, 536 235, 436 4, 436501, 436524, 436528

Abstract:

A system for the detection of ligands comprising at least one receptor and an amplification mechanism coupled to the receptor wherein an amplified signal is produced as a result of receptor binding a ligand. Examples of suitable amplification mechanisms include antibody-embedded liquid crystalline materials; use of alpha-2-macroglobulin to encage an enzyme, whereby the enzyme is separated from its substrate by an receptor; and a receptor engineered to inhibit the active of site of an enzyme only in the absence of a ligand. Also provided are methods for the automatic detection of ligands.

US Patent:

20020052002, May 2, 2002

Filed:

Mar 29, 2001

Appl. No.:

09/821396

Inventors:

Gary Niehaus - Kent OH, US
Christopher Woolverton - Kent OH, US
Oleg Lavrentovich - Kent OH, US
Tomohiro Ishikawa - Cleveland OH, US
Kathleen Doane - Ravenna OH, US

International Classification:

G01N033/53
C12M001/34
G02F001/1333

US Classification:

435/007100, 435/287200, 349/056000

Abstract:

Devices and systems for the detection of ligands comprising at least one receptor and an amplification mechanism comprising a liquid crystalline, where an amplified signal is produced as a result of receptor binding to a ligand are provided. Also provided are methods for the automatic detection of ligands.

US Patent:

20070243593, Oct 18, 2007

Filed:

May 21, 2007

Appl. No.:

11/751108

Inventors:

Christopher Woolverton - Kent OH, US
Gary Niehaus - Kent OH, US
Kathleen Doane - Ravenna OH, US
Steven Schmidt - Akron OH, US
Steven Signs - Hudson OH, US

Assignee:

KENT STATE UNIVERSITY - Kent OH

International Classification:

C12M 1/34
C12M 1/00
C12N 11/00
C12N 11/02

US Classification:

435177000, 435174000, 435283100, 435287100, 435287200

Abstract:

A system for the detection of ligands comprising at least one receptor and an amplification mechanism coupled to the receptor wherein an amplified signal is produced as a result of receptor binding a ligand. Examples of suitable amplification mechanisms include antibody-embedded liquid crystalline materials; use of alpha-2-macroglobulin to encage an enzyme, whereby the enzyme is separated from its substrate by an receptor; and a receptor engineered to inhibit the active of site of an enzyme only in the absence of a ligand. Also provided are methods for the automatic detection of ligands.

US Patent:

61718025, Jan 9, 2001

Filed:

Jun 10, 1998

Appl. No.:

9/095196

Inventors:

Christopher J. Woolverton - Kent OH
Gary D. Niehaus - Kent OH
Kathleen J. Doane - Ravenna OH
Oleg Lavrentovich - Kent OH
Steven P. Schmidt - Akron OH
Steven A. Signs - Hudson OH

Assignee:

Kent State University - Kent OH

International Classification:

G01N 3353
C12P 2106
C07K 100
C07H 2104

US Classification:

435 71

Abstract:

A system for the detection of ligands comprising at least one receptor and an amplification mechanism coupled to the receptor wherein an amplified signal is produced as a result of receptor binding a ligand. Examples of suitable amplification mechanisms include antibody-embedded liquid crystalline materials; use of alpha-2-macroglobulin to encage an enzyme, whereby the enzyme is separated from its substrate by an receptor; and a receptor engineered to inhibit the active of site of an enzyme only in the absence of a ligand. Also provided are methods for the automatic detection of ligands.