Kathleen M Perry

US Patent:

7407747, Aug 5, 2008

Filed:

Oct 15, 2002

Appl. No.:

10/272450

Inventors:

Kathleen M. Perry - San Francisco CA, US
Robert E. Ruhfel - San Francisco CA, US
Sandra L. Spurgeon - San Mateo CA, US
Peter Ma - Cupertino CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 1/68
C07H 21/00
C07H 21/02
C12P 19/34

US Classification:

435 6, 435 911, 435 912, 536 231, 536 253, 536 266

Abstract:

A dry composition comprising a nucleotide linked to a fluorescent dye is provided. A method of making a dry composition comprising the step of drying a solution comprising a nucleotide linked to a fluorescent dye is provided. A method is provided for performing a reaction on a nucleic acid substrate comprising: (a) providing a vessel containing a dried composition comprising a nucleotide linked to a fluorescent dye, and (b) adding to the vessel an aqueous solution and the nucleic acid substrate. A kit is provided for determining the sequence of a nucleic acid, the kit comprising a dry composition comprising a nucleotide linked to a fluorescent dye.

US Patent:

20110257031, Oct 20, 2011

Filed:

Feb 11, 2011

Appl. No.:

13/026046

Inventors:

John BODEAU - San Mateo CA, US
Heinz BREU - Palo Alto CA, US
Kathleen PERRY - San Francisco CA, US
Adam HARRIS - Carlsbad CA, US
Patrick GILLES - Carlsbad CA, US
Miho Gilles - Carsbad CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C40B 30/04
C07H 21/04
C40B 40/06

US Classification:

506 9, 506 16, 536 2431

Abstract:

Provided herein are systems, compositions and methods for tracking, sorting and/or identifying sample polynucleotides using nucleic acid barcodes. The barcodes provided herein are oligonucleotides that are designed to be uniquely identifiable. The nucleic acid barcodes have properties that permit them to be sequenced with high accuracy and/or reduced error rates. In some embodiments, the nucleic acid barcodes are designed to have certain nucleotide sequences that make up overlapping dibase color positions (also called color positions). The order of the overlapping dibase color positions can be determined using fluorophore-encoded dibase probes in a fluorophore color calling scheme to give high fidelity reads.