Ken Han Chan

US Patent:

20180202904, Jul 19, 2018

Filed:

Jan 18, 2018

Appl. No.:

15/874673

Inventors:

- Pasadena CA, US
Ken Y. Chan - Pasadena CA, US
Alon Grinbaum - West Hollywood CA, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

G01N 1/30
G01N 1/36
G01N 1/34
A61K 35/32

Abstract:

Described herein is a bone tissue clearing method with enhanced optical access. Compositions and techniques for bone tissue clearing include continuous convective flow during the clearing process, amino alcohol to minimize tissue autofluorescence, and an imaging procedure that minimizes refractive index variations in light-sheet microscopy. These improvements allowed the Inventors to achieve whole-bone clearing with an imaging depth of up to about 1.5 mm while maintaining fluorescence and a signal-to-noise ratio (SNR) that permits detection and 3D placement of single cells. In various embodiments, the present application teaches methods and kits for clearing and optionally subsequently visualizing tissue containing bone.

US Patent:

20170166926, Jun 15, 2017

Filed:

Dec 9, 2016

Appl. No.:

15/374596

Inventors:

- Pasadena CA, US
Viviana Gradinaru - La Canada - Flintridge CA, US
Ken Y. Chan - Pasadena CA, US

International Classification:

C12N 15/86
C07K 14/005
C12N 15/113
A61K 38/02
C07K 16/18
A61K 38/18
A61K 38/48
A61K 38/50
A61K 38/43
A61K 38/17
A61K 38/20
C12N 7/00
A61K 38/47

Abstract:

Disclosed herein are peptide sequences capable of directing adeno-associated viruses (AAV) to target specific environments, for example the nervous system and the heart, in a subject. Also disclosed are AAVs having non-naturally occurring capsid proteins comprising the disclosed peptide sequences, and methods of using the AAVs to treat diseases.

US Patent:

20160131560, May 12, 2016

Filed:

Nov 6, 2015

Appl. No.:

14/935279

Inventors:

- Pasadena CA, US
Bin Yang - Pasadena CA, US
Jennifer Treweek - Pasadena CA, US
Ken Chan - Pasadena CA, US

Assignee:

CALIFORNIA INSTITUTE OF TECHNOLOGY - Pasadena CA

International Classification:

G01N 1/30

Abstract:

In various embodiments, the present application teaches methods and kits for clearing and optionally subsequently visualizing tissue containing bone. In some embodiments, the method includes serially incubating cleared bone in refractive index matching solutions with progressively higher refractive indexes. In some embodiments, the methods teach immunolabeling and/or staining tissue containing bone and optionally visualizing the immunolabeled and/or stained tissue containing bone.