Kenneth L Owen

US Patent:

20150368634, Dec 24, 2015

Filed:

Jun 12, 2015

Appl. No.:

14/738095

Inventors:

- Peoria IL, US
Kenneth L. Owen - Peoria IL, US
John G. Schierer - Marquette Heights IL, US

International Classification:

C12N 15/10

Abstract:

A method of extracting DNA from a biological sample includes the steps of freezing a biological sample; mechanically breaking down the sample to produce a processed sample; mixing the processed sample with a lysis solution; and cycling the mixture through a series of pressure fluctuations to produce a resulting lysate. The method may further include the steps of exposing the lysate to a material that binds to DNA present in the sample and collecting the DNA from the sample using an elution solution. An apparatus for practicing the method includes a first segment for mechanically breaking down the sample; a second segment for pressurizing a mixture of the processed sample and lysis solution to produce a lysate; and wherein the first segment is fluidly coupled to the second segment. The apparatus may also include a third segment in which the lysate is exposed to the binding material.