Kenneth L Shepard

US Patent:

7880551, Feb 1, 2011

Filed:

Dec 29, 2006

Appl. No.:

12/159750

Inventors:

Steven Chan - Yonkers NY, US
Kenneth L. Shepard - Ossining NY, US
Zheng Xu - Staten Island NY, US

Assignee:

The Trustees of Columbia University in the City of New York - New York NY

International Classification:

G06F 1/00
H03B 5/00
H03B 5/18
H03L 5/00
H03L 7/00
H03L 7/099
H03L 7/24

US Classification:

331 55, 331 2, 331 96, 331117 D, 331179, 331183, 713500, 713503

Abstract:

Systems and methods for distributing a clock signal are disclosed. In some embodiments, systems for distributing a clock signal include a plurality of resonant oscillators, each comprising an inductor; and a differential clock grid that distributes the clock signal. The differential clock grid is coupled to the plurality of resonant oscillators and the clock signal, and the inductances of the inductors are configured such that a resonant frequency of the plurality of resonant oscillators is substantially equal to the frequency of the clock signal.

US Patent:

8263411, Sep 11, 2012

Filed:

Jan 29, 2009

Appl. No.:

12/362071

Inventors:

Rastislav Levicky - Irvington NY, US
Napoleon Tercero - New York NY, US
Kang Wang - Staten Island NY, US
Ping Gong - Cambridge MA, US
Kenneth Shepard - Ossining NY, US

Assignee:

Polytechnic Institute of NYU - Brooklyn NY

International Classification:

G01N 33/50

US Classification:

436 94, 205792, 536 241

Abstract:

A method to monitor the progress of hybridization between nucleic acid strands in solution and Morpholino strands immobilized on a solid support such as a working electrode in-situ, in real-time, and using label-free electrochemical measurements sensitive to hybridization-induced changes in the near-surface dielectric constant and charge organization.

US Patent:

8273578, Sep 25, 2012

Filed:

Mar 18, 2012

Appl. No.:

13/423228

Inventors:

Rastislav Levicky - Irvington NY, US
Napoleon Tercero - New York NY, US
Kang Wong - Staten Island NY, US
Ping Gong - Elmhurst NY, US
Kenneth Shepard - Ossining NY, US

International Classification:

G01N 33/50

US Classification:

436 94, 205792, 536 241

Abstract:

Use of Morpholinos, a class of uncharged DNA analogues, for surface-hybridization applications. Monolayers of Morpholino probes on gold supports are fabricated with methods similar to those employed with DNA, and are used to hybridize efficiently and sequence-specifically with target strands. Hybridization-induced changes in the interfacial charge organization are analyzed with electrochemical methods and compared for Morpholino and DNA probe monolayers. Molecular mechanisms connecting surface hybridization state to the interfacial capacitance are identified and interpreted through comparison to numerical Poisson-Boltzmann calculations. Positive as well as negative capacitive responses (contrast inversion) to hybridization are possible, depending on surface populations of mobile ions as controlled by the applied potential. Quantitative comparison of surface capacitance with target coverage (targets/area) reveals a near-linear relationship, and demonstrates sensitivities (limits of quantification) in the pg mmrange.

US Patent:

8445893, May 21, 2013

Filed:

Jul 19, 2010

Appl. No.:

12/839095

Inventors:

Inanc Meric - New York NY, US
Kenneth Shepard - Ossining NY, US
Noah J. Tremblay - New York NY, US
Philip Kim - New York NY, US
Colin P. Nuckolls - New York NY, US

Assignee:

Trustees of Columbia University in the City of New York - NY

International Classification:

H01L 29/08

US Classification:

257 29, 257 40, 257 43, 257310, 257E2904

Abstract:

An apparatus or method can include forming a graphene layer including a working surface, forming a polyvinyl alcohol (PVA) layer upon the working surface of the graphene layer, and forming a dielectric layer upon the PVA layer. In an example, the PVA layer can be activated and the dielectric layer can be deposited on an activated portion of the PVA layer. In an example, an electronic device can include such apparatus, such as included as a portion of graphene field-effect transistor (GFET), or one or more other devices.

US Patent:

20100035248, Feb 11, 2010

Filed:

Jun 15, 2008

Appl. No.:

12/139477

Inventors:

Rastislav Levicky - Irvington NY, US
Napoleon Tercero - New York NY, US
Ping Gong - New York NY, US
Kenneth Shepard - Ossining NY, US

International Classification:

C12Q 1/68
C12M 1/34

US Classification:

435 6, 4352872

Abstract:

The sequence determination, detection, and quantification of nucleic acid molecules through sequence-specific binding (hybridization) on a solid support, specifically when Morpholinos are used as the surface-immobilized probe species in surface-based nucleic acid assays, and the assays as disclosed herein.

US Patent:

20120164753, Jun 28, 2012

Filed:

Oct 28, 2011

Appl. No.:

13/283670

Inventors:

Matthew Johnston - New York NY, US
Kenneth Shepard - Ossining NY, US
Ioannis Kymissis - New York NY, US

Assignee:

The Trustees of Columbia University in the City of New York - New York NY

International Classification:

G01N 27/00
H03B 5/36
H01L 41/22
H01L 41/04

US Classification:

436501, 310325, 331158, 422 69, 31032321, 422 90, 436149, 29 2535

Abstract:

An apparatus comprises a thin-film bulk acoustic resonator such as including an acoustic mirror, a piezoelectric region acoustically coupled to the acoustic mirror, and first and second conductors electrically coupled to the piezoelectric region. In an example, an integrated circuit substrate can include an interface circuit connected to the first and second conductors of the resonator, the integrated circuit substrate configured to mechanically support the resonator. An example can include an array of such resonators co-integrated with the interface circuit and configured to detect a mass change associated with one or more of a specified protein binding, a specified antibody-antigen coupling, a specified hybridization of a DNA oligomer, or an adsorption of specified gas molecules.

US Patent:

20130180867, Jul 18, 2013

Filed:

Mar 6, 2013

Appl. No.:

13/787341

Inventors:

The Trustees of Columbia University in the City of New York - New York NY, US
Kenneth L. Shepard - Ossining NY, US

Assignee:

The Trustees of Columbia University in the City of New York - New York NY

International Classification:

C12Q 1/00
G01N 27/447
G01N 27/26

US Classification:

2057775, 2057945

Abstract:

A system and method for detecting a single-molecule using an integrated circuit which includes at least one membrane having a nanopore located between first and second reservoirs and a low-noise preamplifier having an electrode formed on the surface thereof is provided. The method includes passing a target molecule through the nanopore, and measuring a current through the nanopore to detect the presence of a biomolecular entity, if any.

US Patent:

20230059683, Feb 23, 2023

Filed:

Jan 7, 2021

Appl. No.:

17/758415

Inventors:

- New York NY, US
Samuel Sternberg - New York NY, US
Siddarth Arumugam - New York NY, US
Kenneth Shepard - New York NY, US

International Classification:

C12N 15/10
C12N 15/113
C12N 9/22
C12Q 1/6897
G01N 33/53
G01N 33/543

Abstract:

The present disclosure provides devices, systems, and methods for detection of nucleic acids based on CRISPR-Cas editing systems, for example for use in biosurveillance. Disclosed herein are systems and methods utilizing two devices: 1) a point of—need disposable “FET Strip” (enzymatic), and 2) an instrument-operated “FET Multiplexor” (electronic), to provide detection of a nucleic acid for biosurveillance.