Kevin Y Cheung

US Patent:

20210140917, May 13, 2021

Filed:

Apr 11, 2019

Appl. No.:

17/045842

Inventors:

- Oakland CA, US
Anne M. Andrews - Los Angeles CA, US
Kevin M. Cheung - Los Angeles CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

G01N 27/414
C12Q 1/6827

Abstract:

A method of detecting and/or discriminating mismatched or complementary nucleic acids using a field-effect transistor (FET). The FET comprises source and drain electrodes formed on substrate and separated by a channel that includes a thin semiconducting film. One or more nucleic acid molecules are immobilized to thin semiconducting film. The FET includes a gate electrode in contact with solution containing the sample (or located on the surface of the device). Samples possibly containing target nucleic acid are exposed to the FET of the biosensor device and current response is measured after samples containing target nucleic acid have been exposed to the FET of the biosensor device. Measured current response in the FET is used to detect and/or discriminate whether target nucleic acid is present as well as complementary or mismatched. Measured current response may also be used to differentiate among different mismatches.