Kevin G Walter

US Patent:

20060127902, Jun 15, 2006

Filed:

Aug 15, 2003

Appl. No.:

10/524432

Inventors:

Stephen Madden - Sudbury MA, US
Clarence Cook - Northboro MA, US
Brian Cook - Northboro MA, US
John Laterra - Baltimore MD, US
Kevin Walter - Pittsburgh PA, US

Assignee:

Genzyme Corporation - Framingham
The John Hopkins University - Baltimore MD

International Classification:

C12Q 1/68
G01N 33/567
G01N 33/53

US Classification:

435006000, 435007200

Abstract:

To gain a better understanding of brain tumor angiogenesis, new techniques for isolating brain endothelial cells (ECs) and evaluating gene expression patterns were developed. When transcripts from brain ECs derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, genes predominantly expressed in the endothelium were identified. Comparison between normal- and tumor-derived endothelium revealed genes that were specifically elevated in tumor-associated brain endothelium. These results confirm that neoplastic and normal endothelium in human brains are distinct at the molecular level, and have significant implications for the development of anti-angiogenic therapies in the future.

US Patent:

20100062002, Mar 11, 2010

Filed:

Nov 11, 2009

Appl. No.:

12/616600

Inventors:

Stephen I. Madden - Sudbury MA, US
Clarence J. Wang - Arlington MA, US
Brian P. Cook - Northboro MA, US
John Laterra - Baltimore MD, US
Kevin Walter - Pittsburgh PA, US

Assignee:

Genzyme Corporation - Framingham MA

International Classification:

A61K 39/395
C12Q 1/68
C40B 30/04
A61K 39/00
A61P 35/00

US Classification:

4241741, 435 6, 506 9, 4241841

Abstract:

To gain a better understanding of brain tumor angiogenesis, new techniques for isolating brain endothelial cells (ECs) and evaluating gene expression patterns were developed. When transcripts from brain ECS derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, genes predominantly expressed in the endothelium were identified. Comparison between normal- and tumor-derived endothelium revealed genes that were specifically elevated in tumor-associated brain endothelium. These results confirm that neoplastic and normal endothelium in human brains are distinct at the molecular level, and have significant implications for the development of anti-angiogenic therapies in the future.