Krishan L Kalra

US Patent:

6451551, Sep 17, 2002

Filed:

May 16, 2000

Appl. No.:

09/573073

Inventors:

Guangrong Zhan - Shrewsbury MA
Krishan L. Kalra - Danville CA
Sheng-Hui Su - San Ramon CA
Taiying Chen - San Ramon CA

Assignee:

BioGenex Laboratories - San Ramon CA

International Classification:

G01N 130

US Classification:

435 4052, 435 405

Abstract:

Releasing the embedding medium from an embedded histochemically reactive tissue specimen is provided by contacting the embedded tissue specimen with a releasing composition under conditions sufficient to release a sufficient portion of the embedding medium associated with the histochemically reactive tissue specimen to permit analysis without substantial adverse effect on the histochemical reactivity of the specimen.

US Patent:

6495106, Dec 17, 2002

Filed:

Dec 15, 2000

Appl. No.:

09/646695

Inventors:

Krishan L. Kalra - Danville CA
Jason Z. Zhang - Dublin CA
Jianghong Shui - San Jose CA

Assignee:

BioGenex Laboratories - San Ramon CA

International Classification:

G01N 2100

US Classification:

422100, 422 99, 422 63, 422 67, 436 46, 436 54, 436174, 436180, 7386401, 118120, 118300

Abstract:

Disclosed is an automated staining apparatus including an arm ( ) moveable in three dimensions, and a hollow tip head ( ) located on the arm including integral reagent tip head ( ), wash tip ( ) and blow tip ( ) for selectively dispensing gas and liquid onto microscope slides. Also disclosed are various sub-components of the apparatus that are specifically adapted to the processing of specimens on slides.

US Patent:

6632598, Oct 14, 2003

Filed:

Mar 11, 1994

Appl. No.:

08/212175

Inventors:

Guangrong Zhang - San Ramon CA
Cheng-Zhi Yu - Pleasant Hill CA
Sheng-Hui Su - San Ramon CA
Krishan L. Kalra - Danville CA
Ding Zhou - Pleasant Hill CA

Assignee:

BioGenex Laboratories - San Ramon CA

International Classification:

C12Q 100

US Classification:

435 4, 435 405, 435267

Abstract:

Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.

US Patent:

7070951, Jul 4, 2006

Filed:

Aug 29, 2003

Appl. No.:

10/652854

Inventors:

Guangrong Zhang - San Ramon CA, US
Cheng-Zhi Yu - Pleasant Hill CA, US
Sheng-Hui Su - San Ramon CA, US
Krishan L. Kalra - Danville CA, US
Ding Zhou - Pleasant Hill CA, US

Assignee:

BioGenex Laboratories - San Ramon CA

International Classification:

G01N 1/30

US Classification:

435 4052, 435 405, 435975

Abstract:

Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.

US Patent:

7472803, Jan 6, 2009

Filed:

Feb 25, 2005

Appl. No.:

11/067657

Inventors:

Krishan L. Kalra - Danville CA, US
Thomas Maxwell - Danville CA, US
Qi Cao - San Leandro CA, US
Michael Huang - San Ramon CA, US
Shahin Iqbal - Danville CA, US

Assignee:

BioGenex Laboratories, Inc. - San Ramon CA

International Classification:

B65G 59/06

US Classification:

221257, 221256, 221263

Abstract:

An automated dispensing assembly includes a base, and a shuttle mounted in the base and movable under automated control between a loading position and a dispensing position. The shuttle includes a cavity configured to carry an object, such as cover. The depth of the cavity in the shuttle is approximately the same (or less) as the thickness of one of the objects. A storage module is mounted proximate the shuttle. The storage module is configured to store a plurality of objects and includes an opening exposing the next object to be dispensed. The cavity is positioned adjacent the opening in the storage module in the loading position and an edge of the cavity separates the object to be dispensed from the other objects in the storage module as the shuttle moves to the dispensing position.

US Patent:

RE40835, Jul 7, 2009

Filed:

Aug 12, 1993

Appl. No.:

11/249180

Inventors:

Shan-Rong Shi - Los Angeles CA, US
Atul K. Tandon - Fremont CA, US
Krishan L. Kalra - Danville CA, US
Nagesh Malhotra - Los Angeles CA, US
Sheng-Hui Su - San Ramon CA, US
Cheng-Zhi Yu - Pleasant Hill CA, US

Assignee:

BioGenx Laboratories, Inc. - San Ramon CA

International Classification:

G01N 33/567

US Classification:

435 721, 435 795, 435 40, 435 52, 436 63, 436175

Abstract:

A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.

US Patent:

20030100043, May 29, 2003

Filed:

Mar 23, 2001

Appl. No.:

09/816849

Inventors:

Krishan Kalra - Danville CA, US
Jason Zhang - San Jose CA, US
Mitchell Lok - Hayward CA, US

Assignee:

BioGenex Laboratories, Inc.

International Classification:

G01N001/30
C12M001/34

US Classification:

435/040500, 435/287300

Abstract:

A device and methods for the automated histotechnological processing of a specimen are disclosed. The device comprises a microwave unit and a specimen positioning device which transports the specimen into a tank within the microwave unit where automated heating takes place. Preferably the microwave unit is controlled by a real-time microwave processor that permits control of the temperature within the microwave tank. Automated methods of dewaxing, antigen retrieval, nucleic acid retrieval, and hematoxylin and eosin staining which can be used in conjunction with the device are also provided. In one embodiment, a method is provided for simultaneous dewaxing and antigen retrieval; solutions useful for this method are also provided.

US Patent:

20030175852, Sep 18, 2003

Filed:

Mar 17, 2003

Appl. No.:

10/381159

Inventors:

Krishan Kalra - Danville CA, US
Jia Jin - San Francisco CA, US

International Classification:

G01N001/30
G01N033/48

US Classification:

435/040500

Abstract:

Methods and compositions for improving in situ hybridization analysis of aldehyde fixed tissue are described.