Krishan K Kalra

US Patent:

RE40835, Jul 7, 2009

Filed:

Aug 12, 1993

Appl. No.:

11/249180

Inventors:

Shan-Rong Shi - Los Angeles CA, US
Atul K. Tandon - Fremont CA, US
Krishan L. Kalra - Danville CA, US
Nagesh Malhotra - Los Angeles CA, US
Sheng-Hui Su - San Ramon CA, US
Cheng-Zhi Yu - Pleasant Hill CA, US

Assignee:

BioGenx Laboratories, Inc. - San Ramon CA

International Classification:

G01N 33/567

US Classification:

435 721, 435 795, 435 40, 435 52, 436 63, 436175

Abstract:

A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.

US Patent:

55784526, Nov 26, 1996

Filed:

Apr 7, 1994

Appl. No.:

8/211595

Inventors:

Shan-Rong Shi - Los Angeles CA
Atul K. Tandon - Fremont CA
Krishan L. Kalra - Danville CA
Nagesh Malhotra - Los Angeles CA
Sheng-Hui Su - San Ramon CA
Cheng-Zhi Yu - Pleasant Hill CA

Assignee:

Biogenex Laboratories - San Ramon CA

International Classification:

G01N 33567

US Classification:

435 721

Abstract:

A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.