Krishna J Fisher

US Patent:

6387368, May 14, 2002

Filed:

Apr 11, 2000

Appl. No.:

09/546738

Inventors:

James M. Wilson - Gladwyne PA
William M. Kelley - La Honda CA
Krishna J. Fisher - New Orleans LA

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

A61K 4800

US Classification:

424 932, 424 931, 424 936, 4352351, 4353201, 435325, 435366, 435369, 435 5, 435 6, 435455, 435456, 435457, 435 914, 435 9141, 435 9142

Abstract:

The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5â and 3â ITR sequences from an AAV, and a selected transgene. Other hybrid vectors form a polycation conjugate and incorporate an AAV rep gene in a single particle. These hybrid virus vectors are characterized by high titer transgene delivery to a host cell and the ability to stably integrate the transgene into the host cell chromosome. Also disclosed is the use of the hybrid vectors to produce large quantities of recombinant AAV.

US Patent:

20010006955, Jul 5, 2001

Filed:

Jan 10, 2001

Appl. No.:

09/757673

Inventors:

James Wilson - Gladwyne PA, US
Krishna Fisher - New Orleans LA, US

International Classification:

A61K031/70
A61K048/00

US Classification:

514/044000, 424/093210

Abstract:

A method of prolonging gene expression by reducing immune response to a recombinant adeno-associated virus (AAV) bearing a desired gene administered into the muscle of a mammal is described.

US Patent:

20020037867, Mar 28, 2002

Filed:

Feb 26, 1999

Appl. No.:

09/242977

Inventors:

JAMES M. WILSON - GLADWYNE PA, US
KRISHNA J. FISHER - NEW ORLEANS LA, US

International Classification:

A61K048/00
C12N015/861

US Classification:

514/044000, 435/069100, 435/320100, 435/455000, 435/456000, 435/457000

Abstract:

A method of prolonging gene expression by reducing immune response to a recombinant adeno-associated virus (AAV) bearing a desired gene administered into the muscle of a mammal is described.

US Patent:

62615513, Jul 17, 2001

Filed:

Dec 5, 1997

Appl. No.:

8/973334

Inventors:

James M. Wilson - Gladwyne PA
Krishna J. Fisher - New Orleans LA
Guang-Ping Gao - Havertown PA

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

A61K 4800
C12N 15861
C12N 1563
C12N 510

US Classification:

424 932

Abstract:

An adenovirus E1/E4 expressing packaging cell line is provided, which permits the generation of recombinant adenoviruses deleted in both gene regions. A method for enhancing the efficiency of transduction of a recombinant AAV into a target cell is provided by infecting a target cell with a recombinant AAV comprising a selected transgene under the control of regulatory sequences. The infected cell is contacted with an agent which facilitates the conversion of single stranded recombinant virus to its double stranded form.

US Patent:

60015578, Dec 14, 1999

Filed:

Aug 25, 1997

Appl. No.:

8/836022

Inventors:

James M. Wilson - Gladwyne PA
Krishna J. Fisher - New Orleans LA
Shu-Jen Chen - Aldan PA
Matthew Weitzman - La Jolla CA

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

C12Q 170
C12N 1586
C12N 1564

US Classification:

435 5

Abstract:

A recombinant adenovirus and a method for producing the virus are provided which utilize a recombinant shuttle vector comprising adenovirus DNA sequence for the 5' and 3' cis-elements necessary for replication and virion encapsidation in the absence of sequence encoding viral genes and a selected minigene linked thereto, and a helper adenovirus comprising sufficient adenovirus gene sequences necessary for a productive viral infection. Desirably, the helper gene is crippled by modifications to its 5' packaging sequences, which facilitates purification of the viral particle from the helper virus.

US Patent:

6203975, Mar 20, 2001

Filed:

Oct 21, 1999

Appl. No.:

9/427048

Inventors:

James M. Wilson - Gladwyne PA
Krishna J. Fisher - New Orleans LA
Shu-Jen Chen - Aldan PA
Matthew Weitzman - La Jolla CA

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

C12Q 168
C12Q 170
C12N 15861
C12N 1564
C12N 510

US Classification:

435 5

Abstract:

A recombinant adenovirus and a method for producing the virus are provided which utilize a recombinant shuttle vector comprising adenovirus DNA sequence for the 5' and 3' cis-elements necessary for replication and virion encapsidation in the absence of sequence encoding viral genes and a selected minigene linked thereto, and a helper adenovirus comprising sufficient adenovirus gene sequences necessary for a productive viral infection. Desirably the helper gene is crippled by modifications to its 5' packaging sequences, which facilitates purification of the viral particle from the helper virus.

US Patent:

58719822, Feb 16, 1999

Filed:

Aug 25, 1997

Appl. No.:

8/836087

Inventors:

James M. Wilson - Gladwyne PA
William M. Kelley - La Honda CA
Krishna J. Fisher - New Orleans LA

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

C12N 1510
C12N 701
C12N 510
C12N 1586

US Classification:

4351723

Abstract:

The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5' and 3'ITR sequences from an AAV, and a selected transgene. Also provided is a hybrid virus linked via a polycation conjugate to an AAV rep gene to form a single particle. These trans-infection particles are characterized by high titer transgene delivery to a host cell and the ability to stably integrate the transgene into the host cell chromosome. Also disclosed is the use of the hybrid vectors and viruses to produce large quantities of recombinant AAV.

US Patent:

58561528, Jan 5, 1999

Filed:

Oct 28, 1994

Appl. No.:

8/331384

Inventors:

James M. Wilson - Gladwyne PA
William M. Kelley - Ann Arbor MI
Krishna J. Fisher - Philadelphia PA

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

C12N 1510
C12N 1564
C12N 1586

US Classification:

4351723

Abstract:

The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5' and 3' ITR sequences from an AAV, and a selected transgene. Other hybrid vectors form a polycation conjugate and incorporate an AAV rep gene in a single particle. These hybrid virus vectors are characterized by high titer transgene delivery to a host cell and the ability to stably integrate the transgene into the host cell chromosome. Also disclosed is the use of the hybrid vectors to produce large quantities of recombinant AAV.