Kristen E Lee

US Patent:

20200406253, Dec 31, 2020

Filed:

Feb 26, 2018

Appl. No.:

16/479729

Inventors:

Deirdre MELDRUM - Phoenix AZ, US
Laimonas KELBAUSKAS - Gilbert AZ, US
Yanqing TIAN - Tempe AZ, US
Honor GLENN - Mesa AZ, US
Clifford ANDERSON - Tempe AZ, US
Kristen LEE - Mesa AZ, US
Ganquan SONG - Mesa AZ, US
Liqiang ZHANG - Chandler AZ, US
Jeff HOUKAL - Los Angeles CA, US
Fengyu SU - Tempe AZ, US
Benjamin UEBERROTH - Scottsdale AZ, US
Jacob MESSNER - Scottsdale AZ, US
Hong WANG - Scottsdale AZ, US
Kimberly BUSSEY - Scottsdale AZ, US

International Classification:

B01L 3/00
G01N 15/14
G02B 21/34

Abstract:

An integrated technological platform enabling real-time quantitative multiparameter metabolic profiling, utilizing either or both of extra and intracellular optical sensors, individually or simultaneously. A scalable embedded micropocket array structure, generally fabricated on fused silica substrates, facilitates the integration of multiple, spatially separated extracellular sensors for multiparameter analysis in a container formed with the use of an activation mechanism forming part of a device configured to hold the container during the measurements. The creation of hermetically sealed microchambers is carried out with a pneumatically and/or mechanically and/or electromechanically driven device that is “floating” within the holding device and that is optionally equipped with a vacuum/suction mechanism to hold a component of the container at its surface.

US Patent:

20180334700, Nov 22, 2018

Filed:

Oct 7, 2016

Appl. No.:

15/757712

Inventors:

- Scottsdale AZ, US
Clifford Anderson - Tempe AZ, US
Honor Glenn - Mesa AZ, US
Kristen Lee - Mesa AZ, US
Mark Richards - Chandler AZ, US
Laimonas Kelbauskas - Gilbert AZ, US
Kimberly Bussey - Phoenix AZ, US
Deirdre Meldrum - Phoenix AZ, US

International Classification:

C12Q 1/24
G01N 33/569

Abstract:

Methods for seeding live cells onto spatially defined regions of a substrate including multiple features (e.g., microwells or other microenvironments) utilize a stencil embodied in a hole-defining sacrificial film. A sacrificial film devoid of holes may be applied over features of a substrate, and a hole generating mechanism (e.g., hot needle or laser) aligned with features may be used to define holes in the film. Alternatively, holes may be predefined in a sacrificial film to form a stencil, and the stencil may be assembled to the substrate with the holes registered with features thereof. Thereafter, cells are seeded through holes in the film. Seeded cells are subject to incubation, further processing, and/or performance of one or more assays, and the hole-defining sacrificial film (stencil) may be removed.

US Patent:

20180264468, Sep 20, 2018

Filed:

Sep 16, 2016

Appl. No.:

15/760984

Inventors:

- Scottsdale AZ, US
Kristen Lee - Mesa AZ, US
Jacob Messner - MESA AZ, US
Laimonas Kelbauskas - Gilbert AZ, US
Benjamin Ueberroth - Tempe AZ, US
Yanqing Tian - Tempe AZ, US
Deirdre Meldrum - Phoenix AZ, US

International Classification:

B01L 3/00

Abstract:

A multi-layer sealing structure for sealing a microwell array defined in or on a substrate includes at least one front compliant layer, a back compliant layer, and a flexural layer arranged between the at least one front compliant layer and the back compliant layer, wherein the at least one front compliant layer is closer than the back compliant layer to microwells of the microwell array. One or more front compliant layers may be optically reflective and/or may embody a sensor layer. The back compliant layer may include an adhesive or various types of rubber, and the flexural layer may include a polymeric material or metal. A multi-layer sealing structure may be separated from a microwell array by peeling. A multi-layer sealing structure allows local disruption of sealing where particle contaminants are present without compromising the sealing performance of an entire microwell array, and without requiring a large sealing force.

US Patent:

20150126404, May 7, 2015

Filed:

Nov 5, 2014

Appl. No.:

14/533349

Inventors:

Yanqing Tian - Tempe AZ, US
Liqiang Zhang - Chandler AZ, US
Fengyu Su - Tempe AZ, US
Deirdre Meldrum - Phoenix AZ, US
Sean Buizer - Tempe AZ, US
Clifford L. Anderson - Tempe AZ, US
Laimonas Kelbauskas - Gilbert AZ, US
Kristen Lee - Mesa AZ, US

International Classification:

G01N 33/50
G01N 21/64
G01N 33/84

US Classification:

506 12, 506 13, 506 39

Abstract:

A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films.