WISCONSIN ALUMNI RESEARCH FOUNDATION - , US Kristin M. Marshall - Plainfield IL, US Sabine Pellett - Madison WI, US Marite Bradshaw - Madison WI, US
Assignee:
WISCONSIN ALUMNI RESEARCH FOUNDATION - Madison WI
International Classification:
C07K 14/33
US Classification:
435 691, 4353201
Abstract:
The present invention provides a novel isolated plasmid, wherein the plasmid is a native plasmid found in unique type A strains and encode either BoNT/A3 or BoNT/A4 and BoNT/B. The present invention also provides a method of obtaining a plasmid-encoded botulinum neurotoxin and botulinum neurotoxin complex comprising the step of isolating a plasmid encoding the cntA/A or cntA/B neurotoxin gene and genes encoding protein components of the toxin complex from a type A strain. The inventors performed comparative analyses of representative BoNT/A subtype strains by pulsed-field gel electrophoresis (PFGE) and Southern hybridizations with probes specific for the BoNT/A and B genes, cntA/A and cntA/B. Unexpectedly, the inventors determined that the genes encoding BoNT/A3 in the A3 strain, and BoNT/A4 and BoNT/B in the A4 strain, are on plasmids.
Plasmid-Encoded Neurotoxin Genes In Clostridium Botulinum Serotype A Subtypes
- Madison WI, US Kristin M. Marshall - Plainfield IL, US Sabine Pellett - Madison WI, US Marite Bradshaw - Madison WI, US
International Classification:
C12N 9/52 C12N 15/74
Abstract:
The present invention provides a novel isolated plasmid, wherein the plasmid is a native plasmid found in unique type A strains and encode either BoNT/A3 or BoNT/A4 and BoNT/B. The present invention also provides a method of obtaining a plasmid-encoded botulinum neurotoxin and botulinum neurotoxin complex comprising the step of isolating a plasmid encoding the cntA/A or cntA/B neurotoxin gene and genes encoding protein components of the toxin complex from a type A strain. The inventors performed comparative analyses of representative BoNT/A subtype strains by pulsed-field gel electrophoresis (PFGE) and Southern hybridizations with probes specific for the BoNT/A and B genes, cntA/A and cntA/B. Unexpectedly, the inventors determined that the genes encoding BoNT/A3 in the A3 strain, and BoNT/A4 and BoNT/B in the A4 strain, are on plasmids.
Ms. Marshall works in Irving, TX and specializes in Neurology. Ms. Marshall is affiliated with Dallas Medical Center, Medical City Dallas Hospital and William P Clements University Hospital.
Microsoft Office Management Microsoft Word Powerpoint Leadership Training Photoshop Education Special Education Music Education Music Production Musical Theatre Acting Project Coordination Event Planning Travel Planning
Blockbuster Inc Sterling, IL Oct 2010 to Apr 2011 Store ManagerRadio Shack Corporation Rockford, IL Jul 2007 to Oct 2010 Store ManagerGuzzardo Music Rockford, IL Sep 2004 to Jan 2006 Sales Associate
Education:
Colorado Technical University Online 2011 Associate of Science in Business AdministrationRochelle Township High School Rochelle, IL 1999 to 2003 Diploma
Blockbuster Inc Sterling, IL Oct 2010 to Apr 2011 Store ManagerRadio Shack Corporation Rockford, IL Jul 2007 to Oct 2010 Store ManagerGuzzardo Music Rockford, IL Sep 2004 to Jan 2006 Sales Associate
Education:
Colorado Technical University Online 2010 to 2012 Associate of Science in Business AdministrationRochelle Township High School Rochelle, IL 1999 to 2003 Diploma
Mar 2012 to 2000 Server/Bartender/TrainerChili's Grill and Bar Aurora, IL Jun 2010 to Mar 2012 Server/To-Go/ExpoT-Mobile Lombard, IL Nov 2006 to Aug 2008 Retail Wireless ConsultantAllstate Insurance Company Woodridge, IL Mar 2002 to Dec 2003 Sr. Customer Insurance ProfessionalEnchanted Castle Lombard, IL Jun 2000 to Jan 2002 Team Leader
Education:
College of Du Page Glen Ellyn, IL 2012 working toward Associate's
Kristin Marshall - - Writer, Editor, Designer, Artist, Internet Fondler - RGB Haus - Modesto, CA - I am a freelance writer and lover of tech, Snuggies, ...
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