Kristin Amanda Marshall

US Patent:

20140065668, Mar 6, 2014

Filed:

Mar 13, 2013

Appl. No.:

13/800997

Inventors:

WISCONSIN ALUMNI RESEARCH FOUNDATION - , US
Kristin M. Marshall - Plainfield IL, US
Sabine Pellett - Madison WI, US
Marite Bradshaw - Madison WI, US

Assignee:

WISCONSIN ALUMNI RESEARCH FOUNDATION - Madison WI

International Classification:

C07K 14/33

US Classification:

435 691, 4353201

Abstract:

The present invention provides a novel isolated plasmid, wherein the plasmid is a native plasmid found in unique type A strains and encode either BoNT/A3 or BoNT/A4 and BoNT/B. The present invention also provides a method of obtaining a plasmid-encoded botulinum neurotoxin and botulinum neurotoxin complex comprising the step of isolating a plasmid encoding the cntA/A or cntA/B neurotoxin gene and genes encoding protein components of the toxin complex from a type A strain. The inventors performed comparative analyses of representative BoNT/A subtype strains by pulsed-field gel electrophoresis (PFGE) and Southern hybridizations with probes specific for the BoNT/A and B genes, cntA/A and cntA/B. Unexpectedly, the inventors determined that the genes encoding BoNT/A3 in the A3 strain, and BoNT/A4 and BoNT/B in the A4 strain, are on plasmids.

US Patent:

20160362671, Dec 15, 2016

Filed:

Aug 25, 2016

Appl. No.:

15/247299

Inventors:

- Madison WI, US
Kristin M. Marshall - Plainfield IL, US
Sabine Pellett - Madison WI, US
Marite Bradshaw - Madison WI, US

International Classification:

C12N 9/52
C12N 15/74

Abstract:

The present invention provides a novel isolated plasmid, wherein the plasmid is a native plasmid found in unique type A strains and encode either BoNT/A3 or BoNT/A4 and BoNT/B. The present invention also provides a method of obtaining a plasmid-encoded botulinum neurotoxin and botulinum neurotoxin complex comprising the step of isolating a plasmid encoding the cntA/A or cntA/B neurotoxin gene and genes encoding protein components of the toxin complex from a type A strain. The inventors performed comparative analyses of representative BoNT/A subtype strains by pulsed-field gel electrophoresis (PFGE) and Southern hybridizations with probes specific for the BoNT/A and B genes, cntA/A and cntA/B. Unexpectedly, the inventors determined that the genes encoding BoNT/A3 in the A3 strain, and BoNT/A4 and BoNT/B in the A4 strain, are on plasmids.