Kristine H Clarke

US Patent:

8119364, Feb 21, 2012

Filed:

May 27, 2010

Appl. No.:

12/789181

Inventors:

Michael D. Burkart - San Diego CA, US
Kristine Clarke - San Diego CA, US
Andrew C. Mercer - LaJolla CA, US
James J. LaClair - San Diego CA, US
Jordan Meier - LaJolla CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12Q 1/34
C12P 21/06
C12N 9/00
C07H 21/04

US Classification:

435 681, 435 18, 435183, 536 232

Abstract:

Methods to generate analogs of coenzyme A in vivo are disclosed. The methods to generate analogs of coenzyme A in a cell comprise reacting pantetheine or a derivative thereof with a reporter to form labeled pantetheine or a derivative thereof, contacting the cell with the labeled pantetheine or derivative thereof such that the labeled pantetheine or derivative thereof enters the cell, phosphorylating the labeled pantetheine or derivative thereof to form phosphopantetheine or a derivative thereof, adenylating the labeled phosphopantetheine or derivative thereof to form a labeled dephosphoCoenzyme A or derivative thereof, and phosphorylating the 3′-hydroxyl of the labeled dephosphoCoenzyme A or derivative thereof to form a labeled coenzyme A analog or derivative thereof.

US Patent:

20070128683, Jun 7, 2007

Filed:

Jul 11, 2006

Appl. No.:

11/485247

Inventors:

Michael Burkart - San Diego CA, US
Kristine Clarke - San Diego CA, US
Andrew Mercer - La Jolla CA, US
James LaClair - San Diego CA, US
Jordan Meier - La Jolla CA, US

International Classification:

C12Q 1/34
C12P 21/06

US Classification:

435018000, 435068100

Abstract:

Methods to generate analogs of coenzyme A in vitro and in vivo are disclosed. The methods comprise reacting pantetheine or a derivative thereof with a reporter to form labeled pantetheine or a derivative thereof, phosphorylating the labeled pantetheine or derivative thereof to form phosphopantetheine or a derivative thereof, adenylating the labeled phosphopantetheine or derivative thereof to form a labeled dephosphoCoenzyme A or derivative thereof, and phosphorylating the 3′-hydrozyl of the labeled dephosphoCoenzume A or derivative thereof to form a labeled coenzyme A analog or derivative thereof.