Kurt Musselmann

US Patent:

20070281882, Dec 6, 2007

Filed:

Jun 5, 2007

Appl. No.:

11/758080

Inventors:

Kurt Musselmann - Tampa FL, US
John R. Hassell - Tampa FL, US
Brad Kane - Lakeland FL, US
Bridgette Alexandrou - Tampa FL, US

Assignee:

UNIVERSITY OF SOUTH FLORIDA - Tampa FL

International Classification:

A61K 38/28
A61K 31/665
A61K 31/375

US Classification:

514 3, 514101, 514474

Abstract:

Provided is a method of stimulating collagen synthesis and proteoglycan (lumican and keratocan) accumulation. Collagenase isolated keratocytes were cultured with or without insulin with or without ascorbate. Insulin stimulates the synthesis of collagen but does not affect the accumulation of lumican and keratocan. Insulin plus ascorbate, however, stimulates the synthesis of collagen and increased the accumulation of these proteoglycans. The accumulation of PGDS, a KSPG that does not interact with collagen, is not affected by ascorbate. Only the collagen made in the presence of ascorbate was pepsin resistant. EDB overrode the effects of ascorbate on pepsin resistance and proteoglycan accumulation.

US Patent:

20110027884, Feb 3, 2011

Filed:

Oct 14, 2010

Appl. No.:

12/904489

Inventors:

Kurt Musselmann - Tampa FL, US
John R. Hassell - Tampa FL, US
Brad Kane - Lakeland FL, US
Bridgette Alexandrou - Tampa FL, US

Assignee:

UNIVERSITY OF SOUTH FLORIDA - Tampa FL

International Classification:

C12N 5/02

US Classification:

435375

Abstract:

Provided is a method of stimulating collagen synthesis and proteoglycan (lumican and keratocan) accumulation. Collagenase isolated keratocytes were cultured with or without insulin with or without ascorbate. Insulin stimulates the synthesis of collagen but does not affect the accumulation of lumican and keratocan. Insulin plus ascorbate, however, stimulates the synthesis of collagen and increased the accumulation of these proteoglycans. The accumulation of PGDS, a KSPG that does not interact with collagen, is not affected by ascorbate. Only the collagen made in the presence of ascorbate was pepsin resistant. EDB overrode the effects of ascorbate on pepsin resistance and proteoglycan accumulation.