Skills:
SPECIALIZED FILEDS/SKILLS -General cloning, BAC recombineering, mutagenesis. -Proficient knowledge, skills and experiences for development and application of genetically engineered mice from vector design, microinjection, embryo transfer, setting up and troubleshoot genotyping and breeding strategy. -Hands on skills in cell culture, establishing stable cell lines, protein purification, biochemical assays (ELISA, HTS assays). -Confocal imaging (vesicle tracking from live cell images), Also developed numerous biophysical parameters of vesicle motility (from establishing formula to generate automated batch processing programs). Mouse in vivo imaging (micron III, light and molecular probe). - Expert in animal physiology and genetics.- Animal skills, such as handling, restraining, anesthesia, microsurgery, oral gavages and injections (IP, IM, IV) in rodents and rabbits. PCR, real time qPCR (allele specific, mis-match), Southern blotting (from setting up to analyzing data) -Isolation and purification of DNA from various tissue and cultural cells including paraffin embedded samples. - Proficiency in data analysis using Excel and statistical software (graph pad, sigma plot and origin plus), image processing (Metamorph, Image J, EZ C1 and Element). -In depth knowledge in following softwares Breeding organization (Labtracks), Statics (Exel, Graphpad, Sigma plot, Origin), Cloning (Vector NTI, encyclone), Imaging (Metamorph, Image J, Element, EZ c-1), Assay (Softmax pro)