Laura J Price

US Patent:

6406871, Jun 18, 2002

Filed:

Oct 15, 1996

Appl. No.:

08/696924

Inventors:

Mark Henry Pausch - Robbinsville NJ
Bradley Alton Ozenberger - Yardley PA
John Richard Hadcock - Mount Holly NJ
Laura Alicia Price - Langhorne PA
Eileen Marie Kajkowski - Ringoes NJ
Donald Richard Kirsch - Princeton NJ
Deborah Tardy Chaleff - Pennington NJ

Assignee:

BASF Aktiengesellschaft - Ludwigshafen

International Classification:

G01N 33569

US Classification:

435 731, 4352542

Abstract:

The present invention is directed to expression vectors and yeast cells transformed therewith containing a first heterologous nucleotide sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second nucleotide sequence which codes for all or a portion of a G protein complex. Said heterologous protein is physically expressed in a host cell membrane in proper orientation for both stereoselective binding of ligands, as well as functional interaction with G proteins on a cytoplasmic side of the cell membrane. In some embodiments, a nucleotide sequence encoding a heterologous or chimeric G protein is expressed in conjunction with nucleotide sequences from the yeast G protein subunits. A second aspect of the present invention provides expression vectors and yeast cells transformed therewith encoding chimeric yeast/heterologous G protein coupled receptors. A third aspect of the present invention is directed to methods of assaying compounds using such expression constructs and yeast cell expression systems to determine the effects of ligand binding to the heterologous receptors expressed in the systems.

US Patent:

6607906, Aug 19, 2003

Filed:

Apr 9, 1998

Appl. No.:

09/057473

Inventors:

Mark Henry Pausch - Robbinsville NJ
Bradley Alton Ozenberger - Yardley PA
John Richard Hadcock - Mount Holly NJ
Laura Alicia Price - Langhorne PA
Eileen Marie Kajkowski - Ringoes NJ
Donald Richard Kirsch - Princeton NJ
Deborah Tardy Chaleff - Pennington NJ

Assignee:

BASF Aktiengesellschaft - Ludwigshafen

International Classification:

G01N 33569

US Classification:

43525411, 435 731, 435 697, 435 695, 4352542, 43525421, 4353201, 436501, 536 235, 530350

Abstract:

A first aspect of the present invention is directed to a transformed yeast cell containing a first heterologous DNA sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second heterologous DNA sequence which codes for a G protein a subunit or portions thereof fused to DNA sequences from the yeast G protein in a subunit. A second aspect of the present invention is a transformed yeast cell containing a heterologous DNA sequence which codes for a G protein coupled receptor. A third aspect of the present invention is a method of assaying compounds to determine effects on cell growth.

US Patent:

7148053, Dec 12, 2006

Filed:

Nov 25, 2002

Appl. No.:

10/302524

Inventors:

Mark Henry Pausch - Robbinsville NJ, US
Bradley Alton Ozenberger - Yardley PA, US
John Richard Hadcock - Mount Holly NJ, US
Laura Alicia Price - Langhorne PA, US
Eileen Marie Kajkowski - Ringoes NJ, US
Donald Richard Kirsch - Princeton NJ, US
Deborah Tardy Chaleff - Pennington NJ, US

Assignee:

BASF Aktiengesellschaft - Ludwigshafen

International Classification:

C12N 1/14
C12N 15/00
C07H 21/04

US Classification:

43525411, 530350, 536 235, 435 697, 435471, 435476, 435483, 4353201, 435 731

Abstract:

Expression vectors and yeast cells that contain a heterologous G protein-coupled receptor gene and a gene mutation that causes increased sensitivity to receptor activation or a gene mutation that permits transcriptional activation of pheromone-responsive genes without cell cycle arrest. Methods of making the yeast cells.

US Patent:

20060110792, May 25, 2006

Filed:

Jul 12, 2005

Appl. No.:

11/178538

Inventors:

Mark Pausch - Robbinsville NJ, US
Laura Price - Langhorne PA, US

International Classification:

C07K 14/705
C07H 21/04
C12P 21/06

US Classification:

435069100, 435320100, 435325000, 530350000, 536023500

Abstract:

This invention relates generally to a new family of potassium channels. More particularly, the present invention relates to the cloning and characterization of a family of distinct trans-membrane potassium ion channels, characterization of such channels, newly identified polynucleotide sequences, polypeptides encoded by such sequences, expression vectors capable of heterologous expression of such polynucleotide sequences, transformed host cells containing the expression vectors, and assay methods and kits therefor for determining the expression of heterologous nucleotide sequences encoding all or a portion of said potassium channels in host cells, chromosome mapping, diagnostic methodologies and kits therefore. Genes encoding potassium channels representative of this family were cloned from human and mouse ESTs, and human brain, heart and kidney cDNA libraries. More particularly, the invention arises in part from the determination that the DNA sequences of these genes encode a structurally distinct potassium channel whose molecular architecture is characterized by four membrane spanning domains and two putative pore forming domains.

US Patent:

20090081764, Mar 26, 2009

Filed:

Dec 12, 2006

Appl. No.:

11/637196

Inventors:

Mark Henry Pausch - Robbinsville NJ, US
Bradley Alton Ozenberger - Yardley PA, US
John Richard Hadcock - Mount Holly NJ, US
Laura Alicia Price - Langhorne PA, US
Eileen Marie Kajkowski - Ringoes NJ, US
Donald Richard Kirsch - Princeton NJ, US
Deborah Tardy Chaleff - Pennington NJ, US

International Classification:

C12N 1/19

US Classification:

4352542

Abstract:

The present invention is directed to expression vectors and yeast cells transformed therewith containing a first heterologous nucleotide sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second nucleotide sequence which codes for all or a portion of a G protein αβγ complex. Said heterologous protein is physically expressed in a host cell membrane in proper orientation for both stereoselective binding of ligands, as well as functional interaction with G proteins on the cytoplasmic side of the cell membrane. In some embodiments, a nucleotide sequence encoding a heterologous or chimeric Gα protein is expressed in conjunction with nucleotide sequences from the yeast G protein βγ subunits. A second aspect of the present invention provides expression vectors and yeast cells transformed therewith encoding chimeric yeast/heterologous G protein coupled receptors. A third aspect of the present invention is directed to methods of assaying compounds using such expression constructs and yeast cell expression systems to determine the effects of ligand binding to the heterologous receptors expressed in the systems.

US Patent:

55590268, Sep 24, 1996

Filed:

Oct 31, 1994

Appl. No.:

8/332312

Inventors:

Laura A. Price - Langhorne PA
Mark H. Pausch - Robbinsville NJ

Assignee:

American Cyanamid Company - Madison NJ

International Classification:

C12N 114
C12N 1500
C07H 2104

US Classification:

4352542

Abstract:

A first aspect of the present invention is the isolation and characterization of invertebrate (i. e. insect and nematode) potassium channel genes. The present invention provides for the isolation of complementary DNA fragments from Drosophila melanogaster and Caenorhabditis elegans which encode conserved amino acid sequence elements unique to the potassium channel gene family. A yeast expression technology is employed to clone cDNAs from Drosophila melanogaster and a hybridization approach is utilized to isolate additional cDNAs from Caenorhabditis elegans. Using the yeast expression technology, a single 2463 base pair cDNA fragment designated Dm ORF1 is isolated.

US Patent:

58468196, Dec 8, 1998

Filed:

Jun 6, 1995

Appl. No.:

8/472045

Inventors:

Mark H. Pausch - Robbinsville NJ
Bradley A. Ozenberger - Yardley PA
John R. Hadcock - Mount Holly NJ
Laura A. Price - Langhorne PA
Eileen M. Kajkowski - Ringoes NJ
Donald R. Kirsch - Princeton NJ
Deborah T. Chaleff - Pennington NJ

Assignee:

American Cyanamid Company - Madison NJ

International Classification:

C12N 1563
C12N 1512
C07K 14705

US Classification:

4353201

Abstract:

The present invention is directed to a expression vectors and yeast cells transformed therewith containing a first heterologous nucleotide sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second nucleotide sequence which codes for all or a portion of a G protein. alpha. beta. gamma. complex. Said heterologous protein is physically expressed in a host cell membrane in proper orientation for both stereoselective binding of ligands, as well as functional interaction with G proteins on the cytoplasmic side of the cell membrane. In some embodiments, a nucleotide sequence encoding a heterologous or chimeric G. alpha. protein is expressed in conjunction with nucleotide sequences from the yeast G protein. beta. gamma. subunits. A second aspect of the present invention provides expression vectors and transformed yeast cells encoding chimeric yeast/heterologous G protein coupled receptors. A third aspect of the present invention is directed to methods of assaying compounds using these expression constructs and yeast cell expression systems to determine the effects of ligand binding to the heterologous receptors expressed in the systems.

US Patent:

56911882, Nov 25, 1997

Filed:

Feb 14, 1994

Appl. No.:

8/195729

Inventors:

Mark Henry Pausch - Robbinsville NJ
Bradley A. Ozenberger - Yardley PA
John Richard Hadcock - Mount Holly NJ
Laura Alicia Price - Langhorne PA
Eileen Marie Kajkowski - Ringoes NJ
Donald Richard Kirsch - Princeton NJ
Deborah Tardy Chaleff - Pennington NJ

Assignee:

American Cyanamid Company - Madison NJ

International Classification:

C12N 1581

US Classification:

4352542

Abstract:

A first aspect of the present invention is directed to a transformed yeast cell containing a first heterologous DNA sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second heterologous DNA sequence which codes for a G protein. alpha. subunit or portions thereof fused to DNA sequences from the yeast G protein a subunit. A second aspect of the present invention is a transformed yeast cell containing a heterologous DNA sequence which codes for a G protein coupled receptor. A third aspect of the present invention is a method of assaying compounds to determine effects on cell growth.