Lawrence W Nicholson

US Patent:

6790669, Sep 14, 2004

Filed:

Dec 14, 2001

Appl. No.:

10/018629

Inventors:

Curtis D. Pfeiffer - Midland MI
Nile N. Frawley - Midland MI
Thomas L. Peters - Midland MI
Philip J. Savickas - Midland MI
David R. Albers - Midland MI
Steven J. Gluck - Lake Jackson TX
Lawrence W. Nicholson - Freeland MI
Jose B. Esquivel H. - Midland MI

Assignee:

The Dow Chemical Company - Midland MI

International Classification:

G01N 3014

US Classification:

436161, 436 63, 436 71, 436 86, 436 94, 436174, 436178

Abstract:

A chemical analysis method for determining chemically related differences between subject biological material such as genetically modified plant material and control biological material such as genetically unmodified plant material, which method includes at least the following six steps. The first step is to contact the subject biological material with a fluid extractant, such as a mixture of water, isopropanol and potassium hydroxide, to produce a fluid extract of the subject biological material. The second step is to contact the control biological material with the fluid extractant to produce a fluid extract of the control biological material. The third step is to chromatograph the fluid extract of the subject biological material, for example, gas or fluid chromatography, to produce a chromatogram of the fluid extract of the subject biological material. The fourth step is to chromatograph the fluid extract of the control biological material to produce a chromatogram of the fluid extract of the control biological material. The fifth step is to determine the differences between the chromatograms, for example, by using the method of U. S. Pat. No.

US Patent:

53670736, Nov 22, 1994

Filed:

Oct 15, 1993

Appl. No.:

8/138613

Inventors:

Bakthan Singaram - Santa Cruz CA
Gary B. Fisher - Boulder Creek CA
Christian T. Goralski - Midland MI
Lawrence W. Nicholson - Midland MI

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07C21300
C07C21508
C07C21338
C07D20712
C07D29508

US Classification:

544170

Abstract:

The present invention relates to a process for the synthesis of chiral enantiomerically pure. beta. -amino alcohols which are extraordinarily important as therapeutic agents for the treatment of a variety of human disorders and as chiral auxiliaries in organic synthesis. The hydroboration of enamines is a versatile and convenient method for the synthesis of both racemic and enantiomerically pure. beta. -amino alcohols in high yields. This methodology enables the synthesis of virtually any. beta. -amino alcohol. Hydroboration of these enamines with chiral organic borohydrides, e. g. either mono- or diisopinocampheylborane, followed by oxidation with aqueous or solid NaOH/30% H. sub. 2 O. sub. 2 or Me. sub. 3 NO, gives the corresponding chiral. beta. -amino alcohol.

US Patent:

56414048, Jun 24, 1997

Filed:

Feb 20, 1996

Appl. No.:

8/596338

Inventors:

Lawrence W. Nicholson - Midland MI
Christian T. Goralski - Midland MI
Curtis D. Pfeiffer - Midland MI

Assignee:

The Dow Chemical Company - Midland MI

International Classification:

B01D 1508

US Classification:

210635

Abstract:

A process for separating enantiomeric mixtures by liquid chromatography using a stationary phase that includes cellulose or amylose derivative and a mobile phase that includes methanol and pentane, the concentration of methanol in the mobile phase being greater than one tenth percent by volume and less than the saturation concentration of methanol in the mobile phase, the concentration of pentane being at least that necessary to resolve the enantiomeric mixture into its enantiomers with a resolution at least one and one half times greater than the pentane of the mobile phase is replaced with hexane.

US Patent:

48371610, Jun 6, 1989

Filed:

Aug 25, 1986

Appl. No.:

6/900038

Inventors:

Timothy S. Stevens - Midland MI
Nile N. Frawley - Midland MI
Daniel J. Swart - Midland MI
William C. Harris - Neshanic Station NJ
Deborah E. Diedering - Sanford MI
Lawrence W. Nicholson - Midland MI
L. David Rothman - Midland MI

Assignee:

The Dow Chemical Company - Midland MI

International Classification:

G01N 3006
G01N 3508

US Classification:

436 52

Abstract:

An analytical chemistry apparatus and method for introducing a reagent into a flowing stream of liquid carrier in order to quantitatively analyze one or more components of a sample added into the carrier. The reagent is permeated across a short section of membrane having relatively large pores. The use of such a membrane: (a) allows the membrane to be protected from physical damage by covering it with a perforate structure; (b) significantly reduces bandspreading across the membrane reagent addition device; (c) reduces the pressure drop across the membrane reagent addition device; and (d) still allows for the permeation of an effective amount of the reagent into the carrier. The reagent is preferably pressurized to minimize leakage of carrier across the membrane and the reagent can be self-pressurized by essentially completely filling the reagent reservoir of the invention with reagent and then hermetically sealing the reservoir.