Leandro G Neves

US Patent:

20130035238, Feb 7, 2013

Filed:

Jun 27, 2012

Appl. No.:

13/535190

Inventors:

Matias Kirst - Gainesville FL, US
Marcio Fernando Ribeiro De Resende, JR. - Gainesville FL, US
Leandro Gomide Neves - Gainesville FL, US
Christopher Dervinis - High Springs FL, US
Kelly Mayrink Balmant - Gainesville FL, US

International Classification:

C40B 50/06
C12N 9/16
C40B 20/00
C07H 21/04

US Classification:

506 2, 506 26, 536 2433, 435196

Abstract:

The present invention provides methods to produce a reduced representation of a genome for sequencing and DNA polymorphism detection. In particular, the invention provides PCR-based methods, with normalization of the amplified products using a duplex-specific nuclease, in order to reduce over-representation of PCR products. Oligonucleotides for use in the disclosed method are also provided.

US Patent:

20220220542, Jul 14, 2022

Filed:

May 13, 2020

Appl. No.:

17/610724

Inventors:

- GAINESVILLE FL, US
LEANDRO GOMIDE NEVES - GAINESVILLE FL, US

International Classification:

C12Q 1/6813
C12Q 1/6806

Abstract:

The disclosure pertains to materials and methods for capturing target genomic regions, comprising cleaving the target genomic region using endonucleases at specific recognition sites having a minimum of between about 10 and about 30 nucleotides and capturing the target genomic region by hybridizing to a bridge oligonucleotide. The disclosure also pertains to analyzing the captured target genomic regions. The endonucleases used for cleavage can be programmable endonucleases that specifically bind to the recognition sites to direct the cleavage. The captured target genomic regions can be amplified, preferably, via polymerase chain reaction or rolling circle amplification and detected or sequenced. Further, the invention pertains to kits for performing the methods of the invention, comprising one or more endonucleases designed to cleave one or more target genomic regions and bridge oligonucleotides designed to capture the one or more target genomic regions. The kits can be customized for specific target genomic regions.

US Patent:

20170283854, Oct 5, 2017

Filed:

Aug 26, 2015

Appl. No.:

15/505568

Inventors:

- Gainesville FL, US
Leandro G. Neves - Gainesville FL, US
Marcio F. Resende - Gainesville FL, US

International Classification:

C12Q 1/68
G06F 19/10
C40B 40/06

Abstract:

The present invention relates to identification and characterization of polymorphisms in a nucleic acid sample. Methods and compositions for the unbiased amplification of multiple target sequences within a nucleic acid sample are provided.

US Patent:

20150133316, May 14, 2015

Filed:

Nov 25, 2014

Appl. No.:

14/553053

Inventors:

- Gainesville FL, US
Marcio Fernando Ribeiro De Resende, JR. - Gainesville FL, US
Leandro Gomide Neves - Gainesville FL, US
Christopher Dervinis - High Springs FL, US
Kelly Mayrink Balmant - Gainesville FL, US

International Classification:

C12N 15/10
C12Q 1/68

US Classification:

506 2, 506 26, 536 2433

Abstract:

The present invention provides methods to produce a reduced representation of a genome for sequencing and DNA polymorphism detection. In particular, the invention provides PCR-based methods, with normalization of the amplified products using a duplex-specific nuclease, in order to reduce over-representation of PCR products. Oligonucleotides for use in the disclosed method are also provided.