Lee N Hoang

US Patent:

20090223893, Sep 10, 2009

Filed:

May 4, 2009

Appl. No.:

12/435381

Inventors:

Douglas T. Gjerde - Saratoga CA, US
Lee Hoang - San Jose CA, US
Chris Suh - San Jose CA, US
Mark W. Abel - San Jose CA, US

International Classification:

B01D 15/08

US Classification:

210635

Abstract:

The invention provides gel filtration columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of gel filtration media positioned above a bottom frit or between a bottom and top frit. In some embodiments, the columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods and devices for desalting and/or buffer exchange of a sample.

US Patent:

20100129873, May 27, 2010

Filed:

Nov 24, 2009

Appl. No.:

12/625535

Inventors:

Chris Suh - San Jose CA, US
Lee Hoang - San Jose CA, US
Douglas T. Gjerde - Saratoga CA, US
David P. Hornby - Widnes, GB

International Classification:

C12P 19/34

US Classification:

435 911

Abstract:

A highly parallel method for gene cloning is presented. PCR products can be isolated using a solid phase and ligated into a positive selection vector. The cloning method has a very high success rate and can be performed entirely by a liquid handling robot with very little human intervention.

US Patent:

20100140173, Jun 10, 2010

Filed:

Feb 21, 2010

Appl. No.:

12/709487

Inventors:

Chris Suh - San Jose CA, US
Lee Hoang - Santa Clara CA, US
Douglas T. Gjerde - Saratoga CA, US

International Classification:

B01D 15/20
B01D 15/22

US Classification:

210656, 2101982

Abstract:

The invention provides gravity chromatographic columns for the automated purification of a material (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of media positioned above a bottom frit or between a bottom and top frit. In some embodiments, the columns employ modified pipette tips as column bodies. In some embodiments, the columns employ modified plates or racks as column bodies. In some embodiments, the invention provides methods and devices for gel filtration, desalting, buffer exchange, ion exchange, ion-pairing, normal phase and reverse phase chromatography. In some embodiments, the invention provides multiplexing gravity flow chromatography on a liquid handling robotic system.

US Patent:

20100170852, Jul 8, 2010

Filed:

Mar 22, 2010

Appl. No.:

12/729103

Inventors:

Chris Suh - San Jose CA, US
Lee Hoang - Santa Clara CA, US
Douglas T. Gjerde - Saratoga CA, US

International Classification:

B01D 15/22

US Classification:

210656, 2101982

Abstract:

The invention provides gravity chromatographic columns for the purification of a material (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of media positioned above a bottom frit or between a bottom and top frit. In some embodiments, the columns employ modified pipette tips as column bodies. In some embodiments, the columns employ modified plates or racks as column bodies. In some embodiments, the invention provides methods and devices for gel filtration, desalting, buffer exchange, ion exchange, ion-pairing, normal phase and reverse phase chromatography. In some embodiments, the invention provides multiplexing gravity flow chromatography on a liquid handling robotic system.

US Patent:

20100200509, Aug 12, 2010

Filed:

Apr 26, 2010

Appl. No.:

12/767659

Inventors:

Chris Suh - San Jose CA, US
Lee Hoang - Santa Clara CA, US
Jennifer E. Grant - Los Gatos CA, US
Douglas T. Gjerde - Saratoga CA, US

International Classification:

B01D 15/04

US Classification:

210670

Abstract:

The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.

US Patent:

20110195518, Aug 11, 2011

Filed:

Feb 8, 2011

Appl. No.:

13/023519

Inventors:

Douglas T. Gjerde - Saratoga CA, US
Lee Hoang - Santa Clara CA, US
Chris Suh - San Jose CA, US
Mark Abel - San Jose CA, US

International Classification:

G01N 1/18
B01L 3/02

US Classification:

436178, 422524

Abstract:

An apparatus and method of using a pipette with pipette tip columns were developed in which a pipette is operated with the pipette tip columns inserted into the wells of a microplate. In this configuration the pipette is free standing and is essentially perpendicular to the microplate. The open lower ends of the pipette tip column are approximately centered within the plate well. The columns and plate are designed in such a way that the open lower ends of the pipette tip columns are in contact with liquid in the plate well however, the columns do not seal on the well bottom, preventing flow in and out of the column. The pipette contains the appropriate firmware and software to control flow for all steps of pipette tip column operation.

US Patent:

20120083598, Apr 5, 2012

Filed:

Sep 30, 2011

Appl. No.:

13/250040

Inventors:

Chris Suh - San Jose CA, US
Lee Hoang - Santa Clara CA, US
Douglas T. Gjerde - Saratoga CA, US

International Classification:

C07H 21/00

US Classification:

536 2542

Abstract:

The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.

US Patent:

20120252115, Oct 4, 2012

Filed:

Mar 29, 2012

Appl. No.:

13/434656

Inventors:

Chris Suh - San Jose CA, US
Lee Hoang - Santa Clara CA, US
Douglas T. Gjerde - Saratoga CA, US

International Classification:

C12N 15/63

US Classification:

4353201

Abstract:

The invention provides pipette tip columns and automated methods for the purification of nucleic acids such as plasmids from unclarified cell lysates containing cell debris as well as methods for making and using such columns. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit.