Lee E Mangiante

Lee Mangiante

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Location:

7437 Tottenham Pl, New Albany, OH 43054

Industry:

Pharmaceuticals

Work:

Eli Lilly and Company Jan 2018 - Jan 2020
Senior Principal Research Associate
Aragen Bioscience, Inc. 2009 - 2009
Intern
University of North Carolina Aug 2004 - Dec 2008
Graduate Research Fellow
Unc Chapel Hill Pathology 2004 - 2008
Graduate Student
University of South Carolina 2002 - 2004
Undergraduate Research Assistant

Education:

University of North Carolina at Chapel Hill School of Medicine 2004 - 2008
Master of Science, Doctorates, Masters, Doctor of Philosophy University of South Carolina 2000 - 2004
Bachelors, Bachelor of Science, Biology

Skills:

Cell Culture
Assay Development
Cell
Molecular Biology
Western Blotting
Cell Biology
Flow Cytometry
Fluorescence Microscopy
Microscopy
Immunohistochemistry
Immunofluorescence
Qpcr
Elisa
Monoclonal Antibodies
Data Analysis
Primary Cells
Angiogenesis
Cell Migration
Cell Signaling
Cancer Biology

Interests:

Gemology
Live Cell Imaging
Baseball
Music
Microscopy
Cytoskeletal Remodeling

Languages:

English
German

Lee Mangiante

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Lee Mangiante New York, NY

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Work:

Eli Lilly and Company

2012 to 2000
Principal Research Associate ImClone Systems
New York, NY
Feb 2011 to 2014
Senior Research Associate ImClone Systems
New York, NY
Jun 2009 to Jan 2011
Research Scientist II/Research Associate Aragen Bioscience
Morgan Hill, CA
Mar 2009 to Mar 2009
Intern University of North Carolina
Chapel Hill, NC
2005 to 2008
Graduate Research Fellow South Carolina Cancer Center
Columbia, SC
2002 to 2004
Undergraduate Research Associate USC School
Columbia, SC
Jun 2001 to Aug 2001
Medicine Undergraduate Research Intern

Education:

University of North Carolina
Chapel Hill, NC
Dec 2008
M.S. in Pathology and Laboratory Medicine University of South Carolina, SC Honors College
Columbia, SC
2004
BS in Biology

Skills:

Cell Biology: Development of cell adhesion assays; Analysis of cell surface proteins by flow cytometry (Intellicyt HTFC and iQue cytometers; BD FACSCalibur, BD FACSAria) Culture of primary (endothelial and vascular smooth muscle) and tumor cells Excellent facility with fluorescent immunocytochemistry, widefield and confocal microscopy in both brightfield and fluorescence settings on fixed and live samples. Received on-site Vendor training to independently operate the Nikon A1 Confocal Microscope. Familiarity with the retinal angiogenesis model; facility with retina dissection, whole-mount staining, and RNA extraction; Generation, cloning, and expansion of stable cell lines; Transfection and microinjection of mammalian cells; Homogenization and downstream molecular analysis of a animal tissues; siRNA design, transfection, and knockdown validation; Isolation of primary VSMCs from mouse aorta; Isolation of primary endothelial cells from human neonatal foreskin; BrDu incorporation, CellTiter Glo, and Caspase Activation Assays; Transwell migration, wound closure and Platypus migration assays; Gravity-enforced multicellular spheroid generation; Endothelial sprouting and aortic ring sprouting assays; Cell path tracking and kymographic analysis Molecular Biology: Affymetrix Quantigene gene expression analysis platform; DNA cloning, purification, and electrophoresis; Standard PCR, RT-PCR, qPCR, and PCR mutagenesis; Primer design and qPCR data analysis; Adenovirus purification; generation of retrovirus from 293T cells; RNA isolation from cells and tissues; Bacterial transformation and culture Protein Sciences: Facility with Mesoscale platform, including development of novel assays; ELISAs (binding, blocking, cytokine quantification); GST protein purification and pulldown, SDS-PAGE, Western and far Western blotting; Antibody labeling and Dynabead conjugation; Immunoprecipitation, co-IP, and cell fractionation; Small GTPase activity assays Scientific Software: Facility with multiple image analysis platforms, including Nikon Elements, Imaris Bitplane, Metamorph, and Volocity; FloJo and Forecyt flow cytometry analysis packages; Web-based databases including NCBI, PubMed, SMART, Ensembl, CCLE, BioGPS, and SABioscience Array Analysis software; Vector NTI, GraphPad Prism (completed in-house training) Familiarity with LIMS tracking system and Sharepoint sites Symyx electronic notebook software