Lee D Spate

US Patent:

20110213198, Sep 1, 2011

Filed:

Aug 28, 2009

Appl. No.:

13/061222

Inventors:

Randall S. Prather - Rocheport MO, US
Rongfeng Li - Hohhot, CN
Clifton N. Murphy - Columbia MO, US
Lee Spate - Columbia MO, US

Assignee:

The Curators of the University of Missouri - Columbia MO

International Classification:

A61B 17/435
C12N 5/07

US Classification:

600 34, 435375

Abstract:

The present invention provides a practical, non-invasive, and efficient method for cryopreservation of an in-vitro-produced porcine embryo. The inventive method treats the NP (such as IVF- or NT-derived) embryo at the one-cell or cleavage stage prior to compaction with high osmolality followed by high speed centrifugation. The high osmolality treatment enlarges the periviteline space, and with centrifugation, enables the separation of the lipids from the cytoplasm. The lipid-separated embryos after high osmolality treatment have been successfully cryopreserved and later recovered and transferred to produce live offspring.

US Patent:

20210017490, Jan 21, 2021

Filed:

Jun 3, 2020

Appl. No.:

16/891776

Inventors:

Ye Yuan - Columbia MO, US
Lee D. Spate - Columbia MO, US
Randall S. Prather - Columbia MO, US
R. Michael Roberts - Columbia MO, US

Assignee:

The Curators of the University of Missouri - Columbia MO

International Classification:

C12N 5/075

Abstract:

The present invention provides a novel oocyte maturation medium or/and embryo culture medium with a chemically defined supplement to produce matured oocytes at high efficiency. The inventive medium or supplement comprises three growth factors, namely, fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF-1) in a synergistic combination. Methods for oocyte and embryo culture are also provided.

US Patent:

20170258495, Sep 14, 2017

Filed:

Feb 28, 2017

Appl. No.:

15/445621

Inventors:

Ye Yuan - Columbia MO, US
Lee D. Spate - Columbia MO, US
Randall S. Prather - Columbia MO, US
R. Michael Roberts - Columbia MO, US

Assignee:

The Curators of the University of Missouri - Columbia MO

International Classification:

A61B 17/435
C12N 5/075

Abstract:

The present invention provides a novel oocyte maturation medium or/and embryo culture medium with a chemically defined supplement to produce matured oocytes at high efficiency. The inventive medium or supplement comprises three growth factors, namely, fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF-1) in a synergistic combination. Methods for oocyte and embryo culture are also provided.