- San Diego CA, US Dmitry K. Pokholok - San Diego CA, US Lena Christiansen - San Diego CA, US
International Classification:
C12Q 1/6848 C12N 15/10 C12Q 1/6874 C12Q 1/6876
Abstract:
Embodiments of systems, methods, and compositions provided herein relate to assays for selectively controlling enzymatic reactions. Some embodiments relate to methods of inhibiting, reducing, or eliminating secondary DNA (such as mitochondrial DNA) sequencing reads from open chromatic sequencing, whole genome sequencing, or targeted sequencing.
- San Diego CA, US Ramesh Ramji - San Diego CA, US Steven Norberg - La Mesa CA, US Lena Christiansen - San Diego CA, US Dmitry K. Pokholok - San Diego CA, US Fan Zhang - San Diego CA, US
Embodiments of systems, methods, and compositions provided herein relate to hollow beads encapsulating single cells. Some embodiments include performing multiple co-assays on a single cell encapsulated within a hollow bead, including nucleic acid sequencing, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.
Analysis System For Orthogonal Access To And Tagging Of Biomolecules In Cellular Compartments
- San Diego CA, US Frank J. Steemers - San Diego CA, US Lena Christiansen - San Diego CA, US Dmitry K. Pokholok - San Diego CA, US Fan Zhang - San Diego CA, US
Assignee:
ILLUMINA, INC. - San Diego CA
International Classification:
C12N 15/10 C12N 9/12 C12N 9/22
Abstract:
The invention relates to a system and methods for enhancing access to nuclear informational molecules, such as DNA, RNA, and proteins, by analytical biomolecules, such as transposome complexes, by treating nuclei with a nuclear permeability enhancer, and to methods of using nuclear membrane, cell membrane, and external compartmentalization approaches as contiguity preserving elements.
Resumes
Senior Research Associate, Advanced Research Group
Illumina - Greater San Diego Area since Jun 2013
Senior Research Associate
Illumina - Greater San Diego Area since Dec 2011
Research Associate II
Illumina - Greater San Diego Area Aug 2011 - Oct 2011
Inside Sales
Illumina - Branford, CT May 2009 - Jul 2011
Research Associate II in Proteomics Development
Affomix Corporation (acquired by Illumina, Inc in 2010) - Branford, CT May 2009 - Jul 2010
Research Assistant of Molecular Sciences
Education:
Denison University 2005 - 2009
Bachelor of Science, Biology
Quinnipiac University 2010
Skills:
Molecular Biology Assay Development Genomics Molecular Cloning Protein Expression Gel Electrophoresis Genetics Pcr Biotechnology Western Blotting Bioinformatics Proteomics Laboratory Automation Dna Sequencing Bio Rad Bioplex 200 System Luminex Xmap Technology Multiplex Genotyping By Minisequencing Protein Purification/Qc Bead Based Assays Pcr/Qpcr/Immuno Pcr Cpt Seq Hiseq2000/2500/4000 Hiseqx Miseq Nextseq Antibodies Sequencing
Interests:
Molecular Biology Cancer Research Documentaries Ht Technologies Genetics Bioinformatics Sequencing Haplotyping Epigenetics See 3 See Less Nat Geo Evo Devo Proteomics Genomics
Languages:
English
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