Leslayann C Schecterson

Molecular Neuroscientist / Research Faculty At University Of Washington

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Position:

In Vivo Transgene Expression Studies of Neuronal Connectivity/Research Faculty at University of Washington

Location:

Greater Seattle Area

Industry:

Higher Education

Work:

University of Washington - Institute for Stem Cell and Regenerative Medicine (ISCRM) since Sep 2012
In Vivo Transgene Expression Studies of Neuronal Connectivity/Research Faculty
University of Washington - Dept of Physiology & Biophysics/Virginia Merrill Bloedel Hearing Research Center May 2001 - Aug 2012
Mechanisms of Neurotrophin Receptor Signaling/Research Scientist
Inologic Inc. (currently ISM Therapeutics) - Greater Seattle Area May 1999 - May 2001
Signaling Mechanisms of Small Molecule Therapeutics/Staff Scientist
University of Washington - Greater Seattle Area 1990 - 1993
Neurotrophin Gene Expression/Postdoctoral research

Education:

University of Washington 1983 - 1989
Ph.D., Pharmacology Bates College 1977 - 1981
B.S., Biology and Chemistry

Languages:

French

Research Lab Supervisor

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Location:

14417 446Th Ave, North Bend, WA 98045

Industry:

Higher Education

Work:

University of Washington Aug 2014 - Feb 2016
Acute Inflammatory-Induced Hyperalgesia: the Cornea As A Live Tissue Model For Drug Discovery
Contract Technical Advisor/Scientific Writer Jun 2014 - Feb 2016
Always Looking To Contribute To New Projects
Seattle Children's Jun 2014 - Feb 2016
Research Lab Supervisor
University of Washington Sep 2012 - Jul 2014
Pioneering the Use of Stem Cells As Therapeutics In the Auditory System
University of Washington Aug 2008 - Aug 2012
In Vivo Transgene Expression Studies of Neuronal Connectivity and Research Scientist

Education:

University of Washington 1983 - 1989
Doctorates, Doctor of Philosophy, Pharmacology Bates College 1977 - 1981
Bachelors, Bachelor of Science, Biology, Chemistry Wethersfield High School
Bangor University

Skills:

Molecular Biology
Cell Culture
Western Blotting
Tissue Culture
Microscopy
Pcr
Biochemistry
Fluorescence Microscopy
Assay Development
Immunohistochemistry
Cell Biology
Protein Purification
Protein Expression
Protein Chemistry
Signal Transduction
Transfection
Confocal Microscopy
In Vitro
Immunoprecipitation
Immunocytochemistry
Immunofluorescence
Neuroscience
Cell
Fluorescence
Cell Signaling
Sds Page
Gel Electrophoresis
Proteomics
Genetics
In Situ Hybridization
In Vivo
Animal Models
Science

Interests:

Skiing
Yoga
Education
Dance
Theatre
Hiking
Playwriting
Science and Technology
Arts and Culture

Languages:

French
Latin
English

Leslayann Schecterson North Bend, WA

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Work:

Dept. of Physiology & Biophysics

2012 to 2000
Junior Research Faculty University of Washington

2001 to 2012
Research Scientist University of Washington
Seattle, WA
2008 to 2011
Research Scientist Inologic, Inc
Seattle, WA
1999 to 2001
Staff Scientist University of Washington

1990 to 1993
Postdoctoral Researcher Dept. of Pharmacology, University of Washington
Seattle, WA
1984 to 1989
Graduate Researcher Harvard Medical School
Boston, MA
1981 to 1983
Research Assistant

Education:

University of Washington
Seattle, WA
1989
Ph.D. in Pharmacology Bates College
Lewiston, ME
1981
B.S. in Chemistry and Biology University College of N. Wales
Bangor
1979 to 1980
Marine Biology and Biochemistry

Skills:

Technical Expertise Mammalian Cell Culture: Stable lines, primary neurons, stable and transient transfection techniques. See Schecterson et al., 1991, 2010; Kanning et al., 2003. In Vivo Expression: Transgene expression in zebrafish, development of miniature high throughput in ovo gene expression system in chick embryos. See Schecterson et al., 2012. Stem Cells: production of chick chimeras using mammalian stem cells. Current projects. Molecular Biology: mRNA isolation, Northern and Southern Blotting, in situ hybridization PCR, cloning, expression vector design and construction, shRNA design and expression. See Schecterson et al., 1991, 1992, 1994, 2010, 2012; Kanning et al., 2003. Protein Expression Analysis: Cell surface biotinylation, pulse-chase labeling, protein palmitoylation detection, protein glycosylation analysis and secretory pathway manipulation, density gradient separation of subcellular membrane fractions, immunolabelling (fluorescent and DAB) of cells in culture and tissue sections, tissue fixation and sectioning, laser capture dissection. See Schecterson et al, 2010, 2012. Kanning et al., 2003. Imaging: Confocal imaging of fixed cells and tissue sections, analysis using Image J, Huygens de-convolution, live imaging of cultured cells expressing fluorescent tagged proteins and organelle-specific dyes. See Schecterson et al., 2012. Proteomics: Laser capture dissection of embryonic brainstem nuclei/membrane preparation for mass spec analysis. Current projects.