Linda E Rich

US Patent:

20140072585, Mar 13, 2014

Filed:

Mar 14, 2013

Appl. No.:

13/831181

Inventors:

Linda E. Rich - Worcester MA, US
Stephen Ming-teh Lu - Woroester MA, US
Natarajan Ramasubramanyan - Westborough MA, US

Assignee:

ABBVIE INC. - North Chicago IL

International Classification:

C07K 1/22

US Classification:

4241771, 5303905

Abstract:

Disclosed herein are compositions and methods for purifying antibody products from a sample matrix. In particular, the present invention relates to compositions and methods for purifying antibody products employing hydrophobic interaction chromatography media. In certain embodiments, the invention provides a method for reducing process-related impurities (e.g., host cell proteins), as well as product-related substances, including molecular weight variants (e.g., aggregates and fragments of the antibody product).

US Patent:

20180222938, Aug 9, 2018

Filed:

Jan 8, 2018

Appl. No.:

15/864378

Inventors:

- North Chicago IL, US
Linda E. Rich - Worcester MA, US
Stephen Ming-Teh Lu - Worcester MA, US
Natarajan Ramasubramanyan - Westborough MA, US

Assignee:

AbbVie Inc. - North Chicago IL

International Classification:

C07K 1/20
C07K 1/36
C07K 1/22
C07K 1/18
C07K 16/24
C07K 1/16

Abstract:

The present invention is directed to methods for purifying a protein of interest, e.g., an antibody, from a sample comprising the protein of interest and at least one impurity, e.g., an aggregate, by employing a hydrophobic interaction chromatography (HIC) method that allows for binding of both the protein of interest and the at least one impurity under strong binding conditions. The present invention is based, at least in part, on the finding that both flow through and bind-elute techniques can be combined to achieve greater purification and recovery of a protein of interest, e.g., an antibody, under isocratic wash conditions and strong binding conditions.

US Patent:

20160115193, Apr 28, 2016

Filed:

Dec 22, 2015

Appl. No.:

14/978553

Inventors:

- North Chicago IL, US
Linda E. Rich - Worcester MA, US
Stephen Ming-teh Lu - Worcester MA, US
Natarajan Ramasubramanyan - Westborough MA, US

International Classification:

C07K 1/22
C07K 16/24
C07K 16/00

Abstract:

Disclosed herein are compositions and methods for purifying antibody products from a sample matrix. In particular, the present invention relates to compositions and methods for purifying antibody products employing hydrophobic interaction chromatography media. In certain embodiments, the invention provides a method for reducing process-related impurities (e.g., host cell proteins), as well as product-related substances, including molecular weight variants (e.g., aggregates and fragments of the antibody product).

US Patent:

20150299249, Oct 22, 2015

Filed:

Dec 11, 2014

Appl. No.:

14/567570

Inventors:

- North Chicago IL, US
Linda E. Rich - Worcester MA, US
Stephen Ming-teh Lu - Worcester MA, US
Natarajan Ramasubramanyan - Westborough MA, US

International Classification:

C07K 1/20
C07K 1/36
C07K 1/18
C07K 1/16
C07K 16/24
C07K 1/22

Abstract:

The present invention is directed to methods for purifying a protein of interest, e.g., an antibody, from a sample comprising the protein of interest and at least one impurity, e.g., an aggregate, by employing a hydrophobic interaction chromatography (HIC) method that allows for binding of both the protein of interest and the at least one impurity under strong binding conditions. The present invention is based, at least in part, on the finding that both flow through and bind-elute techniques can be combined to achieve greater purification and recovery of a protein of interest, e.g., an antibody, under isocratic wash conditions and strong binding conditions.