Ling Tang

US Patent:

20210002716, Jan 7, 2021

Filed:

Jul 13, 2020

Appl. No.:

16/927898

Inventors:

- Grand Cayman, KY
Shengrong LIN - Fremont CA, US
Ling Fung TANG - San Francisco CA, US

International Classification:

C12Q 1/6853
C12Q 1/6858
C12Q 1/68
G16B 25/00

Abstract:

In some aspects, the present disclosure provides methods for enriching amplicons, or amplification products, comprising a concatemer of at least two or more copies of a target polynucleotide. In some embodiments, a method comprises sequencing the amplicons comprising at least two or more copies of a target polynucleotide. In some embodiments, the target polynucleotides comprise sequences resulting from chromosome rearrangement, including but not limited to point mutations, single nucleotide polymorphisms, insertions, deletions, and translocations including fusion genes. In some aspects, the present disclosure provides compositions and reaction mixtures useful in the described methods.

US Patent:

20200010884, Jan 9, 2020

Filed:

May 16, 2017

Appl. No.:

16/301707

Inventors:

- George Town, Grand Cayman, KY
Shengrong LIN - Fremont CA, US
Ling Fung TANG - San Francisco CA, US
Yontao LU - Sunnyvale CA, US
Zhaohui SUN - Albany CA, US
Yingyu WANG - Mountain View CA, US
Li WENG - Fremont CA, US

International Classification:

C12Q 1/6869
C40B 50/06
C12Q 1/6827
C12Q 1/6858

Abstract:

In some aspects, the present disclosure provides methods for identifying sequence variants, as well as methods of determining copy number of a genetic locus in a sample. Systems and kits for performing methods of the disclosure, as well as compositions produced by or useful in methods of the disclosure are also provided. In some embodiments, methods comprise extending 3′ ends of polynucleotides by adding one or more pre-determined nucleotides. In some embodiments, methods comprise use of a strand-tagging sequence.

US Patent:

20180298434, Oct 18, 2018

Filed:

Apr 6, 2018

Appl. No.:

15/947100

Inventors:

- Grand Cayman, KY
Shengrong Lin - Fremont CA, US
Ling Fung Tang - San Francisco CA, US

International Classification:

C12Q 1/6853
C12Q 1/6858

Abstract:

In some aspects, the present disclosure provides methods for enriching amplicons, or amplification products, comprising a concatemer of at least two or more copies of a target polynucleotide. In some embodiments, a method comprises sequencing the amplicons comprising at least two or more copies of a target polynucleotide. In some embodiments, the target polynucleotides comprise sequences resulting from chromosome rearrangement, including but not limited to point mutations, single nucleotide polymorphisms, insertions, deletions, and translocations including fusion genes. In some aspects, the present disclosure provides compositions and reaction mixtures useful in the described methods.