Charles Robert Petrie - Woodinville WA Lisa Marie Rutledge - Brea CA Jerold Randall Morgan - Renton WA
Assignee:
Becton Dickinson and Company - Franklin Lakes NJ
International Classification:
C12Q 168
US Classification:
435 6
Abstract:
An improved method is described for increasing the rate and specificity of hybridization of target polynucleotide sequences with complementary probes. The method uses amphipathic hydrocarbon polymers (AHP), exemplified by polyvinyl sulfonic acid or polystyrene sulfonic acid, to effect the improved hybridization characteristics. The method may be used in solution phase, solid phase, or in situ hybridization formats.