Lisa M Walker

US Patent:

6631570, Oct 14, 2003

Filed:

Feb 7, 2002

Appl. No.:

10/077697

Inventors:

Lisa Walker - Houston TX 77041

International Classification:

A43B 2136

US Classification:

36100, 36 42

Abstract:

A structure of a low to high heel shoe wherein a sole FIG. having two semicircular âDâ shaped punched holes on either side of the shank is formed in the rear of the shoe sole area, to receive a bolt fitting FIG. having a bight slot and two opposing distal members, fitting through the punched holes and over the shoe shank thereby adjoining to the heel FIG.

US Patent:

20190390245, Dec 26, 2019

Filed:

Jun 25, 2019

Appl. No.:

16/451504

Inventors:

- Fibourg, CH
Charlon Tolliver - Forth Worth TX, US
Denise Rodeheaver - Fort Worth TX, US
Stephen Paul Shannon - Arlington TX, US
Fan Zhang - Keller TX, US
Lisa Maddox Walker - Fort Worth TX, US
Kenneth Keven Williams - Kennedale TX, US

International Classification:

C12Q 1/04
C12Q 1/02

Abstract:

A new two-layer modified agar overlay cytotoxicity method for medical device safety assessment are developed. The new two layer modified agar overlay cytotoxicity method has equivalent sensitivity, and greater efficiency compared to the ISO/USP direct contact and agar overlay assays. Assays were carried out in 6-well microplates. Nutrient agar medium with a 0.5% agar concentration was used to provide a base nutrient layer to support cell growth, L929 cells mixed nutrient agar (0.33% agar) were seeded on top of the base agar and cytotoxicity was evaluated after 24 hours per USP. Results demonstrated the two layer modified agar overlay cytotoxicity assay performs as well as the ISO/USP direct contact method with distinct advantages. Results showed that this two layer modified agar overlay method is more promising as compared to the traditional agar overlay and direct contact methods due to the diluted soft agar, avoided potential mechanical damage from test materials, and represents a valuable tool to evaluate medical device cytotoxicity.