Long T Pham

US Patent:

20220298545, Sep 22, 2022

Filed:

Aug 19, 2020

Appl. No.:

17/636590

Inventors:

- Canton MA, US
Tsai-Chin Wu - San Marcos CA, US
Long Kim Pham - San Diego CA, US
Yong Wang - Sharon MA, US
Ming Lei - Sharon MA, US

International Classification:

C12Q 1/6806
C12Q 1/6869
C12Q 1/6876
C12Q 1/6813
C12Q 1/6844

Abstract:

The present disclosure provides methods and compositions for tracking nucleic acid fragment origin by target-specific barcode tagging when original nucleic acid targets break into small fragments. Nucleic acid targets are captured in vitro on a solid support with clonally localized nucleic acid barcode templates. Many nucleic acid targets can be processed simultaneously in a massively parallel fashion without partition. These nucleic acid target tracking methods can be used for a variety of applications in both whole genome sequencing and targeted sequencing in order to accurately identify genomic variants, haplotype phasing and assembly, for example.

US Patent:

20210047683, Feb 18, 2021

Filed:

Feb 8, 2019

Appl. No.:

16/968576

Inventors:

- Canton MA, US
Tsai-Chin WU - San Marcos CA, US
Long Kim PHAM - San Diego CA, US
Yong WANG - Sharon MA, US

International Classification:

C12Q 1/6869
C12Q 1/6804
C12Q 1/6834
C12Q 1/6876

Abstract:

The present disclosure provides methods and compositions for tracking nucleic acid fragment origin by target-specific barcode tagging when original nucleic acid targets break into small fragments. Nucleic acid targets are captured in vitro on a solid support with clonally localized nucleic acid barcode templates. Many nucleic acid targets canbe processed simultaneously in a massively parallel fashion without partition. These nucleic acid target tracking methods can be used for a variety of applications in both whole genome sequencing and targeted sequencing in order to accurately identify genomic variants, haplotype phasing and assembly, for example.

US Patent:

20190078150, Mar 14, 2019

Filed:

Mar 1, 2017

Appl. No.:

16/077295

Inventors:

- Carlsbad CA, US
Long Kim Pham - San Diego CA, US
Junchen Gu - San Diego CA, US
Ming Lei - Sharon MA, US

International Classification:

C12Q 1/6834
C12Q 1/6874
C12Q 1/6876
C40B 40/06

Abstract:

The present disclosure provides methods and kits for tracking nucleic acid target origin by barcode tagging of the targets when they break into smaller fragments. Nucleic acid targets are captured in vitro by clonally localized nucleic acid barcode templates on a solid support. Millions of nucleic acid targets can be processed simultaneously in a massively parallel fashion without additional partition. These captured targets are broken into small fragments, and a target specific barcode sequence is tagged on each fragment as an identification of their original target. These nucleic acid target tracking methods can be used for a variety of applications in both whole genome sequencing and targeted sequencing.