A sensitive intermediate metabolite such as a folic acid derivative or analog is stabilized by complexing it with a complementary binder or receptor such as a protein and then lyophilizing the complex. An example of such a folic acid derivative is N-5-methyltetrahydrofolic acid.
James E. Ellis - Stoughton MA Graham P. Lidgard - Wellesley MA Gerald Odstrchel - Walpole MA Louis J. Riceberg - Needham MA
Assignee:
Corning Glass Works - Corning NY
International Classification:
G01N 3358 G01N 3382 G01N 3350
US Classification:
435 7
Abstract:
A radioassay procedure and reagent kit therefor for detecting auto blocking antibody, such as auto blocking antibody which interferes with the complexation of intrinsic factor with vitamin B. sub. 12. A receptor, i. e. , intrinsic factor, is immobilized on a support and the amount of ligand, i. e. , vitamin B. sub. 12, capable of binding therewith in the presence of a biological fluid sample is determined.
Improvement in a competitive protein binding assay for N-5-methyltetrahydrofolic acid wherein any folate standard used is N-5-methyltetrahydrofolic acid complexed with folate binder protein. The protein of the complex is destroyed prior to the addition of immobilized folate binder protein. The immobilized folate binder protein participates in competitive binding with unknown folate and labeled folate reagents.
BioBridge Strategies, LLC since Mar 2003
Owner
Chiron Diagnostics Jan 1995 - Apr 1999
Vice President
Ciba Corning 1988 - 1992
Executive Director
Ciba Corning Diagnostics Jan 1986 - Dec 1989
Director
Corning Medical Nov 1979 - Dec 1985
Director
Education:
Brandeis University 1964 - 1979
Doctor of Philosophy (Ph.D.), Biochemistry
Skills:
Biotechnology Lifesciences Hardware Diagnostics Medical Devices Start Ups Technology Transfer Oncology Pharmaceutical Industry Molecular Biology R&D Clinical Development Product Development