Philip J. Barr - Berkeley CA Anthony J. Brake - Berkeley CA Randal J. Kaufman - Boston MA Louise Wasley - Medfield MA Polly A. Wong - Mountain View CA
Assignee:
Genetics Institute, Inc. - Cambridge MA Chiron Corporation - Emeryville CA
International Classification:
C12N 1557 C12N 1579 C12N 1585 C12N 964
US Classification:
536 232
Abstract:
Compositions and methods are provided for endopeptidase production and for enhanced efficiencies of processing heterologous precursor polypeptides to mature polypeptides, including proteins requiring gamma-carboxylation for biological activity. These compositions and methods utilize recombinant PACE, a mammalian endopeptidase that is specific for dibasic amino acid sites.
Expression Of Pace In Host Cells And Methods Of Use Thereof
Philip J. Barr - Berkeley CA Anthony J. Brake - Berkeley CA Randal J. Kaufman - Boston MA Louise Wasley - Medfield MA Polly A. Wong - Mountain View CA
Assignee:
Genetics Institute, Inc. - Cambridge MA Chiron Corporation - Emeryville CA
International Classification:
C12N 964 C12N 948 C12N 1557 C12N 1579
US Classification:
435226
Abstract:
Compositions and methods are provided for endopeptidase production and for enhanced efficiencies of processing heterologous precursor polypeptides to mature polypeptides, including proteins requiring gamma-carboxylation for biological activity. These compositions and methods utilize recombinant PACE, a mammalian endopeptidase that is specific for dibasic amino acid sites.
Randal J. Kaufman - Boston MA Charles B. Shoemaker - Belmont MA Louise C. Wasley - West Roxbury MA
Assignee:
Genetics Institute, Inc. - Cambridge MA
International Classification:
C12P 2100 C12P 1934 C12N 1500 C12N 120
US Classification:
435 68
Abstract:
High yields of active Factor IX are produced by culturing a CHO cell line transfected with chromosomally-integrated Factor IX cDNA in medium to which vitamin K is added.
Expression Of Pace In Host Cells And Methods Of Use Thereof
Philip J. Barr - Berkeley CA Anthony J. Brake - Berkeley CA Randal J. Kaufman - Boston MA Louise Wasley - Medfield MA Polly A. Wong - Mountain View CA
Assignee:
Genetics Institute, Inc. - Cambridge MA Chiron Corporation - Emeryville CA
International Classification:
G12N 1500 G12N 1557 G12N 1581 G12N 1585
US Classification:
435 691
Abstract:
Compositions and methods are provided for endopeptidase production and for enhanced efficiencies of processing heterologous precursor polypeptides to mature polypeptides, including proteins requiring gamma-carboxylation for biological activity. These compositions and methods utilize recombinant PACE, a mammalian endopeptidase that is specific for dibasic amino acid sites.