Maria G Costa

Maria Costa - Lawyer

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Specialties:

Litigation
Criminal Defense
Civil Rights
Criminal Defense

ISLN:

922426544

Admitted:

2012

University:

California Western SOL, San Diego, CA; Univ of Arizona, Tucson, AZ

Maria E Costa

Maria Madalena Costa

Maria E. Costa, Hadonfield NJ Realtor Associate, State of New Jersey Realtor Associate

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Specialties:

Buyer's Agent
Listing Agent
Short-Sale
Staging

Work:

Lenny Vermaat & Leonard
90 Tanner Street
(856)4659487 (Office), (856)4659487 (Cell), (866)7467903 (Fax)

Licenses:

1325002 (State of New Jersey Realtor Associate)

Description:

"My commitment to upholding the beliefs and values ofLenny, Vermaat & Leonard, Realtorsis a daily priority. Istrongly believe that my client's success comes from my commitment, honesty and attentiveness. "Your wants and needs in real estate can be vastly different from the next and because of this my focus is to diligently listen to your requests and advise you in a straightforward and sincere manner. "As my client you will be enriched with the knowledge and experience that I have developed through years of experience working in the financial services industry. "Through all these valuable efforts, my commitment to you will bring the most beneficial outcome with all your real estate needs!"

Links:

Site
LinkedIn

Maria Julios Costa

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Specialties:

Psychiatry

Maria Costa San Francisco, CA

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Work:

Stanford University

Oct 2011 to 2000
Staff Researcher Stanford University
Stanford, CA
Sep 2009 to Sep 2011
Postdoctoral scholar Free University of Brussels
Brussels, IL
Oct 2001 to May 2006
Graduate student Institute of Molecular Pathology and Immunology

Sep 2000 to Sep 2001
Undergraduate student

Education:

Free University of Brussels & University of Porto (joint PhD program)
Brussels
2006
Ph.D. in Human Biology University of Coimbra
Coimbra
2001
B.S. in Biochemistry

Skills:

Cell biology: human and mouse cells/stem cells isolation and primary culture. Cell transfection (lipofection and electroporation). Retrovirus and lentivirus production for delivery of shRNA and protein over-expression. Establishment and maintenance of stable cell lines. Multiparametric flow cytometry for immunophenotyping and analysis of apoptosis/cytotoxicity, cell differentiation and proliferation. Fluorescence microscopy. Cell sorting (FACS and MACS) and utilization of FACSDiva and FlowJo softwares. Analysis of stem cell differentiation. Immuno-electron microscopy (TEM). Oncology: genetic and preclinical mouse models. Subcutaneous and orthotopic tumor transplantation. Intraperitoneal injection. In vivo monitoring of tumor survival, tumor initiation, progression and drug response (genetic and preclinical mouse models of breast cancer and B- and T-cell lymphoma). In vivo monitoring of tumor development using molecular imaging. Tissue harvest and processing (frozen and paraffin), histology, immunohistochemistry and immunofluorescence microscopy. Growth in soft agar assay. Functional analysis of tumor angiogenesis. Cell-based assays for validation of cytotoxicity of therapeutic compounds. Molecular Biology: Preparation of genomic DNA, total protein, total and small non-coding RNA, cDNA. PCR. Analysis of gene expression by Northern and Western blotting, RT-qPCR (Taqman and Sybr green), hybridization on microarrays and flow cytometry for intracellular antigens. Chromatin immunoprecipitation (ChIP-qPCR). TA cloning, bacteria transformation and plasmid purification. Transcription reporter assays in vitro (luciferase, beta-galoctosidase) and in vivo (beta-galoctosidase). Utilization of Genespring for analysis of gene expression data sets. Biochemistry: Sub-cellular fractionation by gradient ultra-centrifugation. Native PAGE. In vitro protein synthesis and GST pull down. Co-immunoprecipitation. Quantification of total protein synthesis. Monoclonal antibody production, characterization/screening and epitope mapping. Measurement of enzyme activity by fluorimetry. Quantification of cyclic AMP by radio-immunoassay. Quantification of cellular cholesterol by gas chromatography. Isolation of polysomal RNA. Analysis of mRNA and protein stability. Isolation of cell surface proteins through biotinylation and protein radiolabeling. Assays that I have developed myself: Antibody-based luminescent assay to quantify protein levels in situ. Analysis of gene expression by flow cytometry. In-cell quantification of total protein synthesis FACSorting of mouse adipose stem cells

Maria Costa Yonkers, NY

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Work:

Education:

Maria Costa East Meadow, NY

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Work:

Smoothie King

Aug 2011 to Present
Shift Leader Designing Dreams
Mineola, NY
Jan 2011 to Feb 2011
Sales Associate

Education: