Marilyn A Morris

US Patent:

20010039005, Nov 8, 2001

Filed:

Jan 23, 2001

Appl. No.:

09/767790

Inventors:

Murali Ramanathan - Amherst NY, US
Marilyn Morris - Amherst NY, US

International Classification:

C12Q001/00
G01N033/53
C12Q001/48
C12Q001/52
C12P019/38
C12P019/40
C12P007/10
C12P005/00
C12N001/00

US Classification:

435/004000, 435/007100, 435/015000, 435/016000, 435/087000, 435/088000, 435/165000, 435/166000, 435/805000, 435/808000, 435/815000

Abstract:

The present invention relates to a method of screening for drug binding to serum proteins by: preparing at least two solutions each including a concentration of a serum protein and a concentration of a candidate drug, wherein the concentration of the candidate drug is different for each of the at least two solutions; exposing each of the at least two solutions to a light source; measuring fluorescent emission by the serum protein or a serum protein-candidate drug complex for each of the at least two solutions upon said exposing; and determining whether a change in fluorescence emission is measured for an increased concentration of the candidate drug, wherein the change in fluorescence emission indicates binding of the candidate drug to the serum protein. A kit useful for performing a fluorimetric screening of drug binding to serum proteins is also disclosed.