Mario M Moronne

US Patent:

20030129618, Jul 10, 2003

Filed:

Aug 9, 2002

Appl. No.:

10/215736

Inventors:

Mario Moronne - Berkeley CA, US
Deborah Charych - Albany CA, US
Jon Nagy - Bozeman MT, US

Assignee:

Regents of the University of California - Oakland CA

International Classification:

C12Q001/70
C12Q001/68
G01N033/53
G01N033/543

US Classification:

435/006000, 435/007100, 436/518000, 435/005000

Abstract:

The present invention relates to methods and compositions for the detection of analytes using the fluorescence that occurs in polymeric material in response to selective binding of analytes to the polymeric materials. In particular, the present invention allows for the fluorescent detection of membrane modifying reactions and analytes responsible for such modifications and for the screening of reaction inhibitors.

US Patent:

57563519, May 26, 1998

Filed:

Jan 13, 1997

Appl. No.:

8/783377

Inventors:

Ehud Y. Isacoff - Berkeley CA
Lidia M. Mannuzzu - Berkeley CA
Mario M. Moronne - Berkeley CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 500
C12N 120
C12N 300
C12N 114

US Classification:

435325

Abstract:

The invention provides protein-based sensors which report changes at a cell surface membrane. These sensors comprise an endogenous cell surface protein having a post-translationally generated luminescer at a predetermined residue. In operation, the protein adopts one of a plurality of different interconvertable signal-dependent conformations, whereunder the luminescer provides corresponding different luminescence. The cells comprise transgenes encoding the protein sensor which is translated from such transgenes by the host cell's machinery with natural amino acids and expressed on the cell surface. After surface expression, the cell is contacted with a luminescer generating reagent which generates a luminescer at a predetermined residue of the protein. A wide variety of luminescers and chemistries for generating the selected luminescer at the target residue of the sensor protein may be used.