Mark A Koehler

US Patent:

20020099183, Jul 25, 2002

Filed:

Feb 28, 2001

Appl. No.:

09/797410

Inventors:

Stefanie Pluschkell - Mystic CT, US
Roderick Geldart - West Kingston RI, US
Lewis Ho - Waterford CT, US
Mark Koehler - Germantown MD, US
Centy Okediadi - Franklin CT, US
Stephen Pias - Gales Ferry CT, US
Marie Zhu - East Lyme CT, US
Steven Hawrylik - North Stonington CT, US

International Classification:

C12P021/02
C12N009/99

US Classification:

530/395000, 435/069200, 435/184000

Abstract:

The present invention relates to a method for the preparation of a Neutrophil Inhibitory Factor (NIF) comprising the cultivation of mammalian cells expressing NIF in an animal component-free growth medium. The present invention may be employed in large-scale preparation of NIF. The invention also relates to a method for the preparation of recombinant proteins comprising the cultivation of mammalian cells expressing an exogenous recombinant protein in an animal component-free growth medium.

US Patent:

20040086964, May 6, 2004

Filed:

Nov 7, 2003

Appl. No.:

10/362263

Inventors:

Stefanie Pluschkell - Groton CT, US
Roderick Geldart - West Kingston RI, US
Lewis Ho - Waterford CT, US
Mark Koehler - Gaithersburg MD, US
Centenary Okediadi - Franklin CT, US
Stephen Pias - Gales Ferry CT, US
Marie Zhu - East Lyme CT, US
Steven Hawrylik - N.Stonington CT, US
Matthew Moyle - Houston MA, US

International Classification:

C12P021/02
C12N005/06
C07K014/475

US Classification:

435/069100, 435/320100, 435/325000, 530/397000

Abstract:

The present invention relates to a method for the preparation of a Neutrophil Inhibitory Factor (NIF) comprising the cultivation of mammalian cells expressing NIF in an animal component-free growth medium. The present invention may be employed in large-scale preparation of NIF. The invention also relates to a method for the preparation of recombinant proteins comprising the cultivation of mammalian cells expressing an exogenous recombinant protein in an animal component-free growth medium.