Mark Lee Shenkin

US Patent:

56396660, Jun 17, 1997

Filed:

Jun 27, 1995

Appl. No.:

8/495771

Inventors:

Mark Lee Shenkin - Pembroke Pines FL

Assignee:

Coulter Corporation - Miami FL

International Classification:

G01N 3348

US Classification:

436 63

Abstract:

A process for determining the reticulocyte population in blood samples, which process includes the use of coriphosphine O to stain reticulocytes and which process is particularly suitable for detection by flow cytometry techniques.

US Patent:

54515254, Sep 19, 1995

Filed:

Feb 14, 1992

Appl. No.:

7/836638

Inventors:

Mark L. Shenkin - Pembroke Pines FL
Ronald M. Hamelik - Miami FL
James C. S. Wood - Coral Springs FL

Assignee:

Coulter Corporation - Miami FL

International Classification:

G01N 3348

US Classification:

436 63

Abstract:

A method is provided to ascertain the total number of cells per unit volume of a cell specimen in an apparatus which does not measure the volume of a suspension containing the specimen as it is being analyzed. The method includes mixing a suspension of a known quantity of particles having a first light scatter signal with a known volume of a cell specimen having a second light scatter signal different from the first light scatter signal to obtain a suspension having a concentration of particles per specimen volume, passing each of said particles and each cell in said cell specimen, in turn, through a light beam, each of said particles and cells producing at least one forward light scattering pattern, counting the number of cells and the number of particles in a portion of said suspension, and determining the total number of cells per unit volume of the specimen. In a preferred embodiment of the invention, the method further includes correlating the light scattering pattern and fluorescence signal of the particles to a predetermined ratio which represents a threshold of minimum instrument performance and adjusting, if the predetermined ratio has not been attained, the operation of said instrument, while the particles are within the incident beam of light, until the measured ratio reaches said predetermined ratio whereby the instrument is analyzing both particles and cells in said cell suspension.